Actinotalea soli sp. nov., isolated from Kubuqi Desert soil

文献类型： 外文期刊

第一作者： Du, Jie

作者： Du, Jie;Ping, Weiwei;Chen, Ya;Bai, Pengze;Cui, Siqi;Zhang, Jianli;Pang, Huancheng

作者机构：

关键词： Actinotalea soli sp; nov; polyphasic taxonomy; 16S rRNA gene

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）

ISSN： 1466-5026

年卷期： 2022 年 72 卷 12 期

页码：

收录情况： SCI

摘要： A Gram -stain positive, facultatively anaerobic, motile rod-shaped strain, BY -33T, was isolated from a soil sample obtained from the Kubuqi Desert, PR China. Phylogenetic analysis based on 16S rRNA gene sequence similarity revealed that strain BY -33T was most closely related to the genus Actinotalea, including Actinotalea ferrariae CF5-4T (98.2 % similarity), 'Actinotalea subter-ranea' HO-Ch2T (98.0 %), Actinotalea solisilvae THG-T121T (97.6 %), 'Actinotalea bogoriensis' 69B4T (97.5 %), Actinotalea fermentans MT (97.3 %) and 'Actinotalea carbonis' T26T (97.0 %). The strain grew at 0-37 degrees C (optimum, 28-30 degrees C) and pH 6.0-11.0 (optimum, pH 9.0-10.0) and with 0-8.0 % (w/v) NaCl (optimum, 3.0%) on tryptic soy agar. It had catalase activity, but no oxidase activity. The polar lipids of strain BY -33T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and phosphati-dylinositol mannosides. The major respiratory quinone of strain BY -33T was MK -10 (H4). Its major fatty acids were anteiso-C15:0, anteiso-C15: 1 A and C16:0. The genomic DNA G+C content of strain BY -33T was 73.0 mol% based on total genome calculations. The average nucleotide identity scores between the genomic sequences of strain BY -33T and the other species of the genus Actino-talea were found to be low (ANIm <85.0 %, ANIb <77.0 % and OrthoANIu <78.0 %). Furthermore, the digital DNA-DNA hybridization and average amino acid identity values between strain BY -33T and the closely related species ranged from 20.5 to 21.0% and from 62.2 to 72.2 %, respectively. Based on the results of phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain BY -33T represents a novel species within the genus Actinotalea, for which the name Actinotalea soli sp. nov. is proposed. The type strain is BY -33T (=CGMCC 1.17460T=KCTC 49362T).

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