Characterization of a Recombinant Thermostable Newcastle Disease Virus (NDV) Expressing Glycoprotein gB of Infectious Laryngotracheitis Virus (ILTV) Protects Chickens against ILTV Challenge

文献类型： 外文期刊

第一作者： Zeng, Zhe

作者： Zeng, Zhe;He, Yan;Wang, Zichen;Yao, Lun;Li, Li;Shang, Yu;Wang, Hongcai;Zhang, Rongrong;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Zeng, Zhe;He, Yan;Wang, Zichen;Yao, Lun;Li, Li;Shang, Yu;Wang, Hongcai;Zhang, Rongrong;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Luo, Qingping

作者机构：

关键词： Newcastle disease virus; glycoprotein gB; infectious laryngotracheitis virus; vector vaccine; thermostability

期刊名称：VIRUSES-BASEL （ 影响因子：4.7； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 15 卷 2 期

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收录情况： SCI

摘要： Infectious laryngotracheitis (ILT) and Newcastle disease (ND) are two important avian diseases that have caused huge economic losses to the poultry industry worldwide. Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated a thermostable recombinant NDV (rNDV) expressing the glycoprotein gB (gB) of infectious laryngotracheitis virus (ITLV) based on the full-length cDNA clone of the thermostable TS09-C strain. This thermostable rNDV, named rTS-gB, displayed similar thermostability, growth kinetics, and pathogenicity compared with the parental TS09-C virus. The immunization data showed that rTS-gB induced effective ILTV- and NDV-specific antibody responses and conferred immunization protection against ILTV challenge in chickens. The efficacy of rTS-gB in alleviating clinical signs was similar to that of the commercial attenuated ILTV K317 strain. Furthermore, rTS-gB could significantly reduce viral shedding in cloacal and tracheal samples. Our study suggested that the rNDV strain rTS-gB is a thermostable, safe, and highly efficient vaccine candidate against ILT and ND.

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