Mutation of the sixth amino acid of the Rep protein has no effect on porcine circovirus 2b but enhances porcine circovirus 2d replication in vitro

文献类型： 外文期刊

第一作者： Wu, Xiaoyan

作者： Wu, Xiaoyan;Yang, Ying;Li, Jun;Zhang, Fan;Wu, Xiaoyan;Wang, Shuo;Xin, Changxun;Li, Chen;Shi, Jianli;Peng, Zhe;Liu, Chang;Han, Hong;Yang, Ying;Tian, Yao;Li, Jiaxin;Xu, Shaojian;Li, Jun;Tian, Yao;Li, Jiaxin;Li, Jun

作者机构：

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2021 年 166 卷 11 期

页码：

收录情况： SCI

摘要： Porcine circovirus type 2 (PCV2) is the etiological agent of post-weaning multisystemic wasting syndrome (PMWS). The original prevalent genotype, PCV2a, has been replaced by genotypes 2b and 2d in the swine population worldwide. The Rep protein is critical for viral replication. Comparison of a large number of Rep protein amino acid (aa) sequences showed that three sites distinguish genotype 2b from genotype 2d. In order to analyze the effect of exchanging the amino acids (asparagine and serine) at position 6 in the Rep proteins of PCV2b and PCV2d, two wild-type and two mutant viruses were rescued. Real-time quantitative PCR and a one-step growth curve were used to determine the viral load to assess the replication ability of the rescued viruses. The results showed that there was no significant difference in in vitro performance between the wild-type PCV2b and the mutated virus, while the mutation of PCV2d enhanced viral replication.

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