文献类型： 外文期刊

第一作者： Guo Wen-Bo

作者： Guo Wen-Bo;Yang Jun-Hua;Han Zheng;Chen Hui-Ying;Sun Zhen-Zhen;Zhao Zhi-Hui;Guo Wen-Bo;Chen Hui-Ying;Sun Zhen-Zhen

作者机构：

关键词： Fumonisins;Anion exchange solid phase extraction column;High performance liquid chromatography-tandem mass spectrometry;Livestock and poultry formula feeds

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2015 年 43 卷 3 期

页码：

收录情况： SCI

摘要： A method was developed for the determination of fumonisin B-1(FB1) and fumonisin B-2(FB2) in livestock and poultry formula feeds by high performance liquid chromatography. tandem mass spectrometry (LC-MS/MS. After extracted by acetonitrile. water (50: 50, V /V) and purified with MAX solid phase extraction column, the fumonisins were separated by Thermo C-18 (100 mmx2. 1 mm, 5 mu m) column with 0. 1% formic acid in water and methanol as the mobile phase. Multiple reaction monitoring (MRM) was used to acquire mass spectrometric data under electrospray positive ionization mode (ESI+). The results showed that the linear correlation coefficients (R-2) of fumonisin FB1 and FB2 were all greater than 0. 999 in the range of 1-500 mu g/L. The limits of quantitation (LOQ) were 0. 098 and 0. 197 mu g/L, and the limits of detection (LOD) were 0. 328 and 0. 656 mu g/L, respectively. At different spiked levels, the recoveries of FB1 and FB2 were ranged from 89. 7% to 95. 1%, and the relative standard deviation (RSD) was ranged from 3. 2% to 8. 6%. Additionally, the detection rate reached 98. 11% screening through the established method in the 106 livestock and poultry formula feeds collected from markets. This result indicates that the method is suitable for accurate quantitative analysis of FB1 and FB2 in different complicated livestock and poultry formula feeds.

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