Purification and partial characterization of a novel hemagglutinating glycoprotein from the cultured mycelia of Hericium erinaceus
文献类型: 外文期刊
第一作者: Cui, Feng-Jie
作者: Cui, Feng-Jie;Li, Yun-Hong;Zan, Xin-Yi;Sun, Wen-Jing;Qian, Jing-Ya;Yang, Yan;Cui, Feng-Jie;Sun, Wen-Jing;Zhou, Qian;Yu, Si-Lian
作者机构:
关键词: Hericium erinaceus, Cultured mycelia;Glycoprotein;Hemagglutinating activity;Structural novelty
期刊名称:PROCESS BIOCHEMISTRY ( 影响因子:3.757; 五年影响因子:3.665 )
ISSN: 1359-5113
年卷期: 2014 年 49 卷 8 期
页码:
收录情况: SCI
摘要: HEG-5, a novel glycoprotein with hemagglutinating activity, was firstly isolated and purified from the cultured mycelia of Hericium erinaceus CZ-2. SDS-PAGE, Native-PAGE and MALDI-TOF-MS proved that HEG-5 was a single band with the molecular weight of approximately 14.4 kDa. HEG-5 had the protein: polysaccharide ratio of approximately 10:1 (%/%) and contained ID-glucose, L-rhamnose, D-galactose and D-mannose with a molar ratio of 1.00:1.09:2.45:7.14 in polysaccharide fraction. HEG-5 was an acidic glycoprotein with a PI value of 6.3 and the higher content of acidic amino acids (Asp, 12.42 +/- 0.25% and Glu, 12.24 +/- 0.26%) in protein fraction. FT-IR and NMR spectra revealed that HEG-5 contained the protein and carbohydrate portions with (1 -> 4)-linked beta-galactose residues and beta-linked glucose residues. Circular dichroism (CD) demonstrated that HEG-5 was a beta-sheet predominant glycoprotein. Hemagglutination assay proved it was a thermo-unstable glycoprotein. The HEG-5 structural novelty was finally presented by protein sequencing and modeling by using MALDI-TOF-MS, NCBI blast search and online SWISS-MODEL Workspace service. (C) 2014 Elsevier Ltd. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Bile acids help maintain immune homeostasis and enhance antioxidant capacity upon Vibrio infection in leopard coral grouper
作者:Zhang, Junwei;Yang, Bowen;Shi, Meng;Chen, Songlin;Zhou, Qian;Zhang, Junwei;Zhang, Jiquan;Wang, Shengpeng
关键词:Plectropomus leopardus; BAs; Vibrio harveyi infection; Transcriptomics; Metabolomics
-
Leveraging a phased pangenome for haplotype design of hybrid potato
作者:Cheng, Lin;Wang, Nan;Bao, Zhigui;Yang, Yuting;Wang, Pei;Zhang, Zhiyang;Tang, Die;Zhang, Pingxian;Wu, Yaoyao;Zhou, Yao;Zheng, Yi;Hu, Yong;Lian, Qun;Ma, Zhaoxu;Zhang, Chunzhi;Zhou, Yongfeng;Huang, Sanwen;Cheng, Lin;Lassois, Ludivine;Wang, Nan;Zhou, Yongfeng;Huang, Sanwen;Bao, Zhigui;Zhou, Qian;Guarracino, Andrea;Garrison, Erik;Tang, Die;Wu, Yaoyao;Zhou, Yao;Lucas, William J.;Stein, Nils;Stein, Nils;Staedler, Thomas;Staedler, Thomas
关键词:
-
Nanoliposome based strategy for enhancing bioavailability and antitumor activity of polyphenol from Sanghuangporus vaninii
作者:Liu, Peng;Wang, Yuyang;Zhang, Zhong;Wu, Di;Chen, Wanchao;Li, Wen;Yang, Yan;Liu, Peng;Wang, Yuyang;Zhang, Zhong;Wu, Di;Chen, Wanchao;Li, Wen;Yang, Yan;Liu, Peng;Liu, Junbo;Wang, Weike
关键词:Sanghuangporus vaninii; Nanoliposome; Liver tumor; Polyphenol; Bioavailability
-
Foodborne iron overload induces oxidative stress and causes mitochondrial damage in the liver of grass carp (Ctenopharyngodon idellus)
作者:Huang, Xiaoman;Yang, Yan;Bai, Yanhan;Yang, Shiyi;Zhang, Linpeng;Liu, Lihan;Tao, Junjie;Tu, Chengming;Lin, Li;Qin, Zhendong;Chen, Bing
关键词:iron overload; Lipid peroxidation; Oxidative stress; Mitochondrial damage; Cell death
-
Cell-free hemoglobin released from hemolysis induces programmed cell death through iron overload and oxidative stress in grass carp (Ctenopharyngodon idella)
作者:Song, Jialing;Li, Ningjing;Yang, Yan;Tian, Ye;Lin, Li;Qin, Zhendong;Chen, Bing;Hu, Jiaxiang
关键词:Ctenopharyngodon idella; Hemoglobin; Iron overload; Oxidative damage
-
Changes of bacterial necromass and their roles in humus conversion during organic wastes composting from different sources
作者:Chen, Wenjie;Yang, Yan;Chang, Su;Wei, Yuquan;Wu, Zhen;Tang, Kaiyan;Chang, Yuan;Zhang, Yifan;Li, Ji;Xu, Ting;Chen, Wenjie;Liang, Xia;Wei, Yuquan;Tang, Kaiyan;Li, Ji;Xu, Ting;Liang, Xia
关键词:Composting; Organic wastes from different sources; Bacterial community; Microbial necromass; Humic acid; Humification
-
Hemolysis-associated release of hemoglobin induces mitochondrial oxidative phosphorylation (OXPHOS) disturbance and aggravates cell oxidative damage in grass carp (Ctenopharyngodon idella)
作者:Yang, Yan;Li, Ningjing;Song, Jialing;Tian, Ye;Li, Jiangtao;Lin, Li;Qin, Zhendong;Chen, Bing
关键词:Hemolysis; Hemin; Mitochondrial respiratory chain; Ctenopharyngodon idella