Metabolic Analysis of Indole-degradated Metabolites in Two Acinetobacter spp.

文献类型： 外文期刊

第一作者： Tesso, T. A.

作者： Tesso, T. A.;Tesso, T. A.;Liu, G.

作者机构：

关键词： Acinetobacter; indole; metabolites; extracellular enzymes

期刊名称：APPLIED BIOCHEMISTRY AND MICROBIOLOGY （ 影响因子：0.8； 五年影响因子：1.1 ）

ISSN： 0003-6838

年卷期： 2023 年 59 卷 4 期

页码：

收录情况： SCI

摘要： Indole and its derivatives are typical nitrogen heterocyclic compounds and have been of immense concern since they are known for the risk of their toxic, recalcitrant, and carcinogenic properties for human, animals and ecological environment. Bacteria play vital role in the degradation and biotransformation of such persistent organic and harmful pollutants. In this experiment, Acinetobacter towneri NTA1-2A and Acinetobacter guillouiae TAT1-6A were used to degrade indole. Indole-degradated metabolites were analyzed by metabolomics analysis of fermentation broth of these strains using LC-MS/MS. The metabolomics analysis showed indole-5-carbonitrile 3-benzoyl (C16H10N2O), dimethyl sulfoxide (C2H6OS), deoxyguanosine (C10H13N5O4), Leu (C6H13NO2), and N-nitroso-pyrrolidine were indole-degradated metabolites formed by A. towneri NTA1-2A while neurine (C5H13NO) and norvaline (C5H11NO2) are common metabolites of both strains. The two strains' degradation metabolites differed from those reported in other studies, demonstrating the diversity of the distinct microbial strains approach to the metabolism of indolic compounds. Extracellular enzymes released to culture medium by these bacteria were also identified. In conclusion, the two bacterial strains have a potential of converting notorious indole compound to non-toxic intermediates mediated by enzymes. Hence, future study should focus on biochemical pathway and genetic basis for indole-degradated metabolites in the two strains.

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