Genotyping by Genome Reducing and Sequencing for Outbred Animals

文献类型： 外文期刊

第一作者： Chen, Qiang

作者： Chen, Qiang;Ma, Yufang;Yang, Yumei;Chen, Zhenliang;Liao, Rongrong;Xie, Xiaoxian;Wang, Zhen;He, Pengfei;Zhang, Xiangzhe;Wang, Qishan;Pan, Yuchun;Yang, Yumei;Tu, Yingying;Yang, Changsuo;Yang, Hongjie;Yu, Fuqing;Zheng, Youmin;Zhang, Zhiwu;Pan, Yuchun

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2013 年 8 卷 7 期

页码：

收录情况： SCI

摘要： Next-generation sequencing (NGS) approaches are widely used in genome-wide genetic marker discovery and genotyping. However, current NGS approaches are not easy to apply to general outbred populations (human and some major farm animals) for SNP identification because of the high level of heterogeneity and phase ambiguity in the haplotype. Here, we reported a new method for SNP genotyping, called genotyping by genome reducing and sequencing (GGRS) to genotype outbred species. Through an improved procedure for library preparation and a marker discovery and genotyping pipeline, the GGRS approach can genotype outbred species cost-effectively and high-reproducibly. We also evaluated the efficiency and accuracy of our approach for high-density SNP discovery and genotyping in a large genome pig species (2.8 Gb), for which more than 70,000 single nucleotide polymorphisms (SNPs) can be identified for an expenditure of only $80 (USD)/sample.

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