Efficient plant regeneration in three cultivars of Hami melon [Cucumis melo L. ssp melo convar. ameri (Pang.) Greb] via organogenesis
文献类型： 外文期刊
第一作者： Liu, L. Z.
作者： Liu, L. Z.;Chen, Y. Y.;Zhu, W. M.;Shi, B.;Chitrampalam, P. R.;Zhai, W. Q.
作者机构：
期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）
ISSN： 1462-0316
年卷期： 2013 年 88 卷 4 期
页码：
收录情况： SCI
摘要： Hami melon [Cucumis melo L. ssp. melo convar. ameri (Pang.) Greb] is reputed to be the 'king of melons', but commercial development of transgenic Hami melon cultivars is limited by low levels of plant regeneration and a high incidence of polyploid regenerants. Regeneration systems for three Hami melon varieties, namely 'Jin Queen', 'Hongmibao', and 'Huangzhuixian', were developed via organogenesis in tissue culture. Leaf explants ranging from 0.125 - 0.500x the full-size of each leaf were prepared in vitro from seedlings of different ages, then cultured on Murashige and Skoog (MS) medium containing various concentrations of 6-benzylaminopurine (BAP; 1.0 - 9.0 mg l(-1)) to determine optimal conditions for induction of adventitious shoots. Leaves from 8-d old seedlings were cut into four, approximately-equal segments. Plant regeneration was greatest on MS medium containing 3.0 mg l(-1) BAP, with a shoot induction frequency of 90% for 'Hongmibao'. For 'Jin Queen' and 'Huangzhuixian', the highest frequencies of shoot induction (86% and 88%, respectively) were obtained on MS medium supplemented with 4.5 mg l(-1) BAP. Adventitious shoots were then elongated on MS medium containing 0.1 mg l(-1) BAP, but rooted successfully on hormone-free MS medium. Approx. 88% of the regenerated plants were diploid, as determined by flow cytometry.
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