The potential mechanism of Bursal-derived BPP-II on the antibody production and avian pre-B cell

文献类型: 外文期刊

第一作者: Feng, Xiuli

作者: Feng, Xiuli;Cao, Ruibing;Zhou, Bin;Liu, Ke;Liu, Xiaodong;Zhang, Yuanpeng;Gu, Jinyan;Chen, Puyan;Liu, Qingtao;Miao, Denian

作者机构:

关键词: BPP-II;Antibody production;Gene microarray;Homologous recombination

期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )

ISSN: 0264-410X

年卷期: 2013 年 31 卷 11 期

页码:

收录情况: SCI

摘要: The bursa of Fabricius is critical for the normal development of the B lymphocytes responsible for antibody production. However, the mechanism of the bursal-derived bioactive factor on B cell development is little reported. In this paper, chicks were immunized with BPP-II and AIV vaccine or AIV antigen, and antibody and IL-4 production were detected. The results showed that BPP-II played strongly inducing roles on the humoral immune responses. To investigate the gene expression at transcriptional level, avian preB lymphocyte DT40 cells were treated with BPP-II, and were analyzed with the gene microarray. The results proved that BPP-II treatment regulated 11 pathways, in which homologous recombination is a vital mechanism which is involved in antibody Ig gene conversion and diversification during B cell development. These results suggested Bursal-derived biological active factor BPP-II might be involved in the antibody production processes and B cell development, which is vital to the humoral central immune organ, the bursa of Fabricius. (C) 2012 Elsevier Ltd. All rights reserved.

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