Rapid and sensitive quantitation of zearalenone in food and feed by lateral flow immunoassay

文献类型： 外文期刊

第一作者： Liu, Gang

作者： Liu, Gang;Han, Zheng;Nie, Dongxia;Yang, Junhua;Zhao, Zhihui;Song, Suquan;Wu, Aibo;Liu, Gang;Zhang, Jingbo;Li, Heping;Liao, Yucai;Song, Suquan;De Saeger, Sarah

作者机构：

关键词： Zearalenone;Lateral flow immunoassay;Quantitation;Food;Feed

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2012 年 27 卷 1 期

页码：

收录情况： SCI

摘要： A lateral flow immunoassay (LFA) for rapid and sensitive quantitation of zearalenone (ZEN) in food and feed stuffs was developed, based on the availability of a new highly sensitive anti-ZEN monoclonal antibody. Compared to previously reported LFA, the established method allows quantitative determination of ZEN within the desirable dynamic range of 1-50 ng mL(-1), corresponding to the recorded color intensity (Cl) values of 65-9, with an IC50 value of 15 ng mL(-1). The developed approach was then applied to a sample collection of thirty two cereal-based baby food, maize, wheat and feed samples, revealing that seven positive samples contained ZEN at concentrations ranging from 20 to 94 mu g kg(-1). The accuracy of the obtained results was confirmed by in-parallel LC-MS/MS analyses. The described assay format is capable of simple, rapid, cost-effective and robust on-site screening or remote quantitative analysis for ZEN, fulfilling the currently strictest regulation requirements for ZEN monitoring worldwide. (C) 2012 Elsevier Ltd. All rights reserved.

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