Characterization and expression of a novel holotype insecticidal crystal protein gene from native Bacillus thuringiensis BM59-2
文献类型: 外文期刊
第一作者: Zheng, Aiping
作者: Zheng, Aiping;Zhu, Jun;Wang, Lingxia;Li, Shuangcheng;Deng, Qiming;Wang, Shiquan;Yu, Xiumei;Guan, Peng;Liang, Hongxia;Li, Ping;Zheng, Aiping;Zhu, Jun;Wang, Lingxia;Li, Shuangcheng;Deng, Qiming;Wang, Shiquan;Yu, Xiumei;Guan, Peng;Liang, Hongxia;Li, Ping;Tan, Furong
作者机构:
关键词: Bacillus thuringiensis;Cry52Ba1;characterization and expression;insecticidal activity
期刊名称:CANADIAN JOURNAL OF MICROBIOLOGY ( 影响因子:2.419; 五年影响因子:2.35 )
ISSN: 0008-4166
年卷期: 2010 年 56 卷 2 期
页码:
收录情况: SCI
摘要: We characterized a novel holotype cry gene (cry52Ba1) harbored in a Bacillus thuringiensis isolate BM59-2 native to China that expresses a spherical crystal protein of about 80 kDa. In this study, the full length of the cry52Ba1 gene was cloned from this strain. Sequence analysis of the gene was also performed. Furthermore, cry52Ba1 was expressed in Escherichia coli BL21(DE)pLysS, and the resulting insecticidal activity showed that the Cry52Ba1 protein exhibited high larvicidal activity against Aedes aegypti (Diptera), with a lethal concentration 50 of 1.526 mu g/mL (95% confidence: 0.740- 3.499 mu g/mL).
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