Integrity of foot-and-mouth disease virions bound to the cells

文献类型: 外文期刊

第一作者: Yi, J.

作者: Yi, J.;Liu, Ch.

作者机构:

关键词: Foot-and-mouth disease virus;virus-cell binding;RNase A;RT-PCR;VP1 protein

期刊名称:ACTA VIROLOGICA ( 影响因子:1.162; 五年影响因子:1.255 )

ISSN: 0001-723X

年卷期: 2010 年 54 卷 4 期

页码:

收录情况: SCI

摘要: In this study, we examined the integrity of Foot-and-mouth disease virus (FMDV) particles during their binding to the surface of BHK-21 cells under physiological condition. For monitoring of the virus integrity we used blocking of the endocytosis with dynasore and cytochalasin D followed by RT-PCR for viral protein VP1 and the resistance of FMDV to the treatment of RNase A. Our results showed that integrin binding to VP1 did not cause a substantial conformational change in the viral capsid. Furthermore, treatment with RNase A showed no effect on the infectivity of intact as well as cell-bound virions. Our findings confirmed that FMDV entered the host cells in the form of intact virions.

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