Full genomic sequence analysis of swine genotype 3 hepatitis E virus isolated from Shanghai

文献类型: 外文期刊

第一作者: Si, Fu-sheng

作者: Si, Fu-sheng;Zhu, Yu-min;Dong, Shi-juan;Yu, Shui-sheng;Yu, Rui-song;Shen, Shi-yuan;Li, Zhen;Zhu, Yu-min;Dong, Shi-juan;Yu, Shui-sheng;Yu, Rui-song;Shen, Shi-yuan;Li, Zhen;Si, Fu-sheng;Yang, Qian

作者机构:

关键词: Hepatitis E virus;Genotype 3;Full genomic sequence;Phylogenetic analysis;Swine viral isolates

期刊名称:VIRUS RESEARCH ( 影响因子:3.303; 五年影响因子:3.445 )

ISSN: 0168-1702

年卷期: 2009 年 144 卷 1-2 期

页码:

收录情况: SCI

摘要: The full genomic nucleotide sequence of a previously identified genotype 3 hepatitis E virus (HEV), strain SAAS-JDY5, was obtained using RT-PCR and rapid amplification of cDNA ends (RACE). The genome consisted of 7225 nucleotides, excluding a poly-A tail at the 3' terminus, and contained three open reading frames (ORFs), ORF-1, ORF-2 and ORF-3, encoding 1702, 660 and 113 amino acids, respectively. Phylogenetic analysis confirmed that SAAS-JDY5 belonged to genotype 3 HEV and was most closely related to the Japanese isolate wbJYG1 (AB222184). SAAS-JDY5 shared approximately 87% nucleotide similarity to human and swine strains from the United States, compared with 74-75% similarity to Asian (genotype 4) and Mexican strains (genotype 2). Alignment of the SAAS-JDY5 genomic sequence with reference sequences of the same genotype revealed one nucleotide substitution and one deletion at positions 5145 and 7189 (3' UTR), respectively. Moreover, SAAS-JDY5 contained two additional nucleotides (AC) at the very end of the T-terminus preceding the poly-A tail of the genome. Comparison of the putative amino acid sequence encoded by the SAAS-JDY5 genome with sequences of other genotype 3 isolates revealed 15 unique amino acid substitutions and one deletion in ORF-1, and three substitutions in ORF-2. (C) 2009 Elsevier B.V. All rights reserved.

分类号:

  • 相关文献

[1]Molecular characterization of Japanese encephalitis viruses circulating in pigs and mosquitoes on pig farms in the Chinese province of Henan. Teng, Man,Luo, Jun,Wang, Xing-Tao,Zhang, Gai-Ping,Fan, Jian-Ming,Yao, Wu,Chen, Lu,Wang, Chuan-Qing.

[2]Molecular detection of hepatitis E virus in sheep from southern Xinjiang, China. Wu, Junyuan,Jin, Meilin,Wu, Junyuan,Jiang, Chunyu,Li, Tao,Si, Fusheng.

[3]Prevalence of hepatitis E virus and porcine caliciviruses in pig farms of Guizhou province, China. Hua, Xiuguo,Shen, Quan,Shen, Quan,Zhang, Wen,Ren, Rongqing. 2011

[4]Seroprevalence of hepatitis E virus infection in psychiatric patients and control subjects in Shandong Province, eastern China. Cong, Wei,Zhu, Xing-Quan,Cong, Wei,Qian, Ai-Dong,Zhu, Xing-Quan,Meng, Qing-Feng,Li, Bing,Ma, Feng-Ling,Wang, Xiang-Yang,Yu, Chang-Zheng. 2014

[5]Molecular epidemiology of hepatitis E virus infections in Shanghai, China. Zhu, Yumin,Si, Fusheng,Yu, Xiaoming,Yu, Ruisong,Dong, Shijuan,Huang, Fenfen,Li, Zhen,Zhu, Yumin,Yu, Ruisong,Dong, Shijuan,Si, Fusheng,Yu, Xiaoming,Zhang, Yuanshu,Cao, Dianjun,Huang, Fenfen. 2011

[6]Determination of the full-genome sequence of hepatitis E virus (HEV) SAAS-FX17 and use as a reference to identify putative HEV genotype 4 virulence determinants. Zhu, Yumin,Yu, Ruisong,Dong, Shijuan,Si, Fusheng,Li, Zhen,Zhu, Yumin,Yu, Ruisong,Dong, Shijuan,Si, Fusheng,Li, Zhen,Yu, Xiaoming,Zhang, Yuanshu,Huang, Fenfen. 2012

[7]Genotype-specific acquisition, evolution and adaptation of characteristic mutations in hepatitis E virus. Ikram, Aqsa,Hakim, Mohamad S.,Zhou, Jian-hua,Wang, Wenshi,Peppelenbosch, Maikel P.,Pan, Qiuwei,Ikram, Aqsa,Hakim, Mohamad S.,Zhou, Jian-hua. 2018

[8]Identification of genotype 4 Hepatitis E virus binding proteins on swine liver cells. Zhang, Wen,Hua, Xiuguo,Yang, Shixing,Cui, Li,Zhang, Wen,Shen, Quan,Yin, Hong. 2011

[9]Seroprevalence of Hepatitis E Virus in Four Species of Parrots in China. Zhang, Xiao-Xuan,Zhang, Nian-Zhang,Zhou, Dong-Hui,Tian, Wei-Peng,Xu, Ying-Tian,Zhu, Xing-Quan,Zhang, Xiao-Xuan,Tian, Wei-Peng,Zhu, Xing-Quan. 2014

[10]Seroprevalence of Hepatitis E Virus Among Pregnant Women and Control Subjects in China. Cong, Wei,Chen, Jia,Zhu, Xing-Quan,Cong, Wei,Qian, Ai-Dong,Zhu, Xing-Quan,Sui, Jian-Chao,Zhang, Xiang-Yan.

[11]Molecular Characterization and Phylogenetic Analysis of New Variants of the Porcine Epidemic Diarrhea Virus in Gansu, China in 2012. Tian, Yufei,Liu, Yuxiu,Yang, Zhenguo,Tian, Yufei,Yu, Zhijun,Huang, Jing,Xin, Yue,Li, Yuanguo,Wang, Tiecheng,Huang, Geng,Feng, Na,Yang, Songtao,Gao, Yuwei,Xia, Xianzhu,Yu, Zhijun,Fan, Shengtao,Yu, Zhijun,Fan, Shengtao,Cheng, Kaihui. 2013

[12]Biotype status and distribution of Bemisia tabaci (Hemiptera : Aleyrodidae) in shandong province of China based on mitochondrial DNA markers. Chu, D.,Jiang, T.,Liu, G. X.,Jiang, D. F.,Tao, Y. L.,Fan, Z. X.,Zhou, H. X.,Bi, Y. P.. 2007

[13]The introduction of the exotic Q biotype of Bemisia tabaci from the Mediterranean region into China on ornamental crops. Chu, D,Zhang, YJ,Brown, JK,Cong, B,Xu, BY,Wu, QJ,Zhu, GR. 2006

[14]Characterization of complete mitochondrial genome of Dezhou donkey (Equus asinus) and evolutionary analysis. Sun, Yan,Jiang, Qiang,Yang, Chunhong,Wang, Xiuge,Ju, Zhihua,Huang, Jinming,Zhong, Jifeng,Wang, Changfa,Tian, Fang,Wang, Yinchao,Zhou, Xiangshan,Ma, Yong.

[15]Genome-wide identification and characterization of SnRK2 gene family in cotton (Gossypium hirsutum L.). Zhao Liu,Xiaoyang Ge,Zuoren Yang,Li, Fuguang,Zhang, Xueyan,Chaojun Zhang,Ge Zhao,Eryong Chen,Ji Liu,Xueyan Zhang,Fuguang Li. 2017

[16]Diagnosis of Peste des Petits Ruminants in Wild and Domestic Animals in Xinjiang, China, 2013-2016. Li, J.,Li, L.,Wu, X.,Liu, F.,Zou, Y.,Wang, Q.,Liu, C.,Bao, J.,Wang, Z.,Wang, W.,Ma, W.,Lin, H.,Huang, J.,Zheng, X.. 2017

[17]A Novel AP2/ERF Transcription Factor CR1 Regulates the Accumulation of Vindoline and Serpentine in Catharanthus roseus. Gao, Fangyuan,Ren, Juansheng,Lu, Xianjun,Ren, Guangjun,Wang, Rui. 2017

[18]Genome-wide analysis of the homeodomain-leucine zipper (HD-ZIP) gene family in peach (Prunus persica). Zhang, C. H.,Ma, R. J.,Shen, Z. J.,Yu, M. L.,Sun, X.,Korir, N. K.. 2014

[19]Genome-Wide Identification and Expression Profile of Dof Transcription Factor Gene Family in Pepper (Capsicum annuum L.). Wu, Zhiming,Bang, Guansheng,Cheng, Jiaowen,Cui, Junjie,Hu, Kailin,Xu, Xiaowan,Luo, Xirong,Chen, Xiaocui,Tang, Xiangqun,Qin, Cheng,Qin, Cheng. 2016

[20]Isolation and phylogenetic analysis of novel gamma-gliadin genes in genus Dasypyrum. Liu, C.,Yang, Z. J.,Yang, E. N.. 2013

作者其他论文 更多>>