Molecular identification and the complete nucleotide sequence of TYLCV isolate from Shanghai of China

文献类型： 外文期刊

第一作者： Zhang Yongping

作者： Zhang Yongping;Zhu Weimin;Wan Yanhui;Zhu Longying;Yu Li;Hui Zhang;Qiu Yang;Sha Kun;Zhang Yongping;Cui Huimei

作者机构：

关键词： molecular identification;complete nucleotide sequence;TYLCV;isolate;Shanghai;DNA-A

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2008 年 36 卷 3 期

页码：

收录情况： SCI

摘要： Tomato yellow leaf curl virus (TYLCV) belongs to the genus Begomovirus, family Geminiviridae. It is transmitted by the whitefly Bemisia tabaci. A pair of primers was designed according to the specific sequence of the TYLCV gene and used in the PCR detection of the virus in the infected tissues of tomato grown in the Shanghai area of China. DNA was extracted from leaves, fruits, seeds, and roots of infected tomato plants separately. The results showed that the target fragment of about 570 bp could be isolated from the leaves, fruits, roots of infected tomato plants, but not from the seeds. Thus, the PCR-based detection technology for the Shanghai TYLCV (TYLCV-Sh10) has been established. Primers were then designed based on the sequence of the 570 bp fragment to obtain the complete DNA-A sequence of TYLCV-Sh10 by genome walking. Sequencing results indicated that the DNA-A sequence of TYLCV-Sh10 contained 2781 nt that included six ORFs. BLAST results showed that DNA-A of TYLCV-Sh10 had low homology with the characterized TYLCV in China (TYLCCNV) except TYLCV-ZJ8 (TYLCV isolated from Zhejiang China). But it was most closely related to TYLCV-USA (99.28% sequence identity). TYLCV-Sh10, TYLCV-ZJ8, TYLCV-USA, TYLCV-Mex, and TYLCV-Eg formed an independent branch by pairwise comparison and phylogenetic analysis. All these results strongly suggested that Sh10 was an isolate of America or Africa TYLCV.

分类号：

  • 相关文献

[1]Genome-wide analysis of bHLH transcription factor and involvement in the infection by yellow leaf curl virus in tomato (Solanum lycopersicum). Wang, Jinyan,Hu, Zhongze,Zhao, Tongmin,Yang, Yuwen,Chen, Tianzi,Yang, Mali,Yu, Wengui,Zhang, Baolong. 2015

[2]Complete nucleotide sequence of pBMB67, a 67-kb plasmid from Bacillus thuringiensis strain YBT-1520. Bao Qiyu,Song Fuping,Sun Ming,Huang Dafang,Liu Guiming,Yu Ziniu. 2007

[3]Molecular characterization of a new begomovirus infecting Senecio scandens in China. Zhou, XP,Cai, JH,Li, GX. 2005

[4]Malvastrum yellow vein virus, a new Begomovirus species associated with satellite DNA molecule. Zhou, XP,Xie, Y,Peng, Y,Zhang, ZK.

[5]Molecular characteristics and evolutionary analysis of field Marek's disease virus prevalent in vaccinated chicken flocks in recent years in China. Yu, Zu-Hua,Chi, Jia-Qi,Hu, Bo,Liu, Ju-Xiong,Yu, Zu-Hua,Teng, Man,Luo, Jun,Yu, Le-Le,Su, Jing-Wei,Chi, Jia-Qi,Zhao, Pu,Hu, Bo,Zhang, Gai-Ping,Yu, Zu-Hua,Ding, Ke,Luo, Jun,Wang, Xin-Wei,Su, Jing-Wei.

[6]Isolation and full-genome sequence of two reticuloendotheliosis virus strains from mixed infections with Marek's disease virus in China. Bao, Ke-yan,Zhang, Yan-ping,Zheng, Hui-wen,Lv, Hong-chao,Gao, Yu-long,Wang, Jing-fei,Gao, Hong-lei,Qi, Xiao-le,Cui, Hong-yu,Wang, Yong-qiang,Ren, Xian-gang,Wang, Xiao-mei,Liu, Chang-jun,Wang, Xiao-mei.

[7]Complete nucleotide sequence of a potyvirus causing maize dwarf mosaic disease in central China. Liu, X,Wang, X,Zhao, Y,Zheng, C,Zhou, G.

[8]Quantifying annual changes in built-up area in complex urban-rural landscapes from analyses of PALSAR and Landsat images. Qin, Yuanwei,Xiao, Xiangming,Dong, Jinwei,Zhang, Geli,Zhang, Yao,Wang, Jie,Wu, Xiaocui,Xiao, Xiangming,Chen, Bangqian,Chen, Bangqian,Liu, Fang.

[9]Shanghai Greenhouse Vegetable Production and Agriculture Tour Experience. Zhu Weimin,Chen Youyuan,Yang Shaojun,Wan Yanhui. 2008

[10]Shanghai Vegetable Supply and Local Production. Zhu Weimin,Wan Yanhui,Yang Shaojun,Zhu Longying. 2012

[11]Town-based spatial heterogeneity of nutrient balance and potential pollution risk of land application of animal manure and fertilizer in Shanghai, China. Qian, Xiaoyong,Shen, Genxiang,Guo, Chunxia,Wang, Zhenqi,Yao, Zheng,Xu, Sixin. 2012

[12]Prevalence of Toxoplasma gondii antibodies, circulating antigens and DNA in stray cats in Shanghai, China. Wang, Quan,Jiang, Wei,Chen, Yong-Jun,Liu, Chun-Ying,Shi, Jin-lei,Li, Xin-tong. 2012

[13]Study on the feeding habits of fishery organisms in the Yangtze River estuary and the southern Yellow Sea in spring with delta C-13 method. Li, Zhongyi,Zhuang, Zhimeng,Dai, Fangqun,Jin, Xianshi. 2011

[14]A novel technique for isolating orchid mycorrhizal fungi. Zhu, G. S.,Yu, Z. N.,Gui, Y.,Liu, Z. Y.,Zhu, G. S.,Gui, Y.,Zhu, G. S.,Gui, Y.. 2008

[15]Molecular identification of the genus Thryssa based on DNA barcoding. Ma, C. -Y.,Ma, H. -Y.,Ni, Y.,Wang, W.,Ma, L. -B.. 2015

[16]Advances in molecular identification, taxonomy, genetic variation and diagnosis of Toxocara spp.. Chen, Jia,Zhou, Dong-Hui,Xu, Min-Jun,Huang, Si-Yang,Li, Ming-Wei,Zhu, Xing-Quan,Chen, Jia,Nisbet, Alasdair J.,Li, Ming-Wei,Wang, Chun-Ren,Zhu, Xing-Quan,Zhu, Xing-Quan.

[17]The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in a tea germplasm repository. Chen, L,Gao, QK,Chen, DM,Xu, CJ.

[18]Detection of Contarinia nasturtii (Diptera : Cecidomyiidae) in New York, a new pest of cruciferous plants in the United States. Kikkert, Julie R.,Hoepting, Christine A.,Wu, Qingjun,Wang, Ping,Baur, Robert,Shelton, Anthony M..

[19]One stop shop: backbones trees for important phytopathogenic genera: I (2014). Hyde, Kevin D.,Mortimer, Peter E.,Xu, Jian-Chu,Hyde, Kevin D.,Mortimer, Peter E.,Xu, Jian-Chu,Hyde, Kevin D.,Ariyawansa, Hiran A.,Dissanayake, Asha J.,Goonasekara, Ishani D.,Jayawardena, Ruvishika S.,Liu, Jian-Kui,Maharachchikumbura, Sajeewa S. N.,Manamgoda, Dimuthu S.,Udayanga, Dhanushka,Hyde, Kevin D.,Ariyawansa, Hiran A.,Dissanayake, Asha J.,Goonasekara, Ishani D.,Jayawardena, Ruvishika S.,Liu, Jian-Kui,Maharachchikumbura, Sajeewa S. N.,Manamgoda, Dimuthu S.,Udayanga, Dhanushka,Hyde, Kevin D.,Nilsson, R. Henrik,Hyde, Kevin D.,Alias, S. Aisyah,Maharachchikumbura, Sajeewa S. N.,Blair, Jaime E.,Cai, Lei,Zhou, Nan,de Cock, Arthur W. A. M.,Dissanayake, Asha J.,Jayawardena, Ruvishika S.,Li, Xinghong,Yan, JiYe,Gorczak, Michal,Pawlowska, Julia,Wrzosek, Marta,Hahn, Matthias,van Kan, Jan A. L.,Terhem, Razak B.,Levesque, C. Andre,Rintoul, Tara L.,Spies, Christoffel F. J.,Laurence, Matthew H.,Summerell, Brett A.,Martin, Frank N.,McKenzie, Eric H. C.,Nair, Prakash V. R..

[20]Molecular identification and expression of the Foxl2 gene during gonadal sex differentiation in northern snakehead Channa argus. Wang, Dan-Dan,Zhang, Gui-Rong,Wei, Kai-Jian,Ji, Wei,Gardner, Jonathan P. A.,Yang, Rui-Bin,Wang, Dan-Dan,Zhang, Gui-Rong,Wei, Kai-Jian,Ji, Wei,Gardner, Jonathan P. A.,Yang, Rui-Bin,Gardner, Jonathan P. A.,Chen, Kun-Ci.

作者其他论文 更多>>

微信小程序