Ectopic expression of OsLFL1 in rice represses Ehdl by binding on its promoter
文献类型: 外文期刊
第一作者: Peng, Ling-Tao
作者: Peng, Ling-Tao;Shi, Zheng-Ying;Li, Lin;Shen, Ge-Zhi;Zhang, Jing-Liu
作者机构:
关键词: OsLFL1;RY cis-element;Ehdl;expression;rice
期刊名称:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( 影响因子:3.575; 五年影响因子:3.381 )
ISSN: 0006-291X
年卷期: 2007 年 360 卷 1 期
页码:
收录情况: SCI
摘要: 133 domain was identified as a novel DNA-binding motif specific to higher plant species. The B3 proteins play important roles in plant development. In the mutant W378, the mutant gene coding OsLFL1, a putative 133 transcription factor gene, was ectopically expressed. In this study, it was found that the flowering promoting gene Ehd1 and its putative downstream genes were all repressed by OsLFL1. Electrophoretic mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP) analyses suggest that OsLFL1 binds to the RY cis-elements (CATGCATG) in the promoter of the Ehd1 gene. Thus, ectopically expressed OsLFL1 might repress Ehd1 via binding directly to the RY cis-elements in its promoter. (c) 2007 Elsevier Inc. All rights reserved.
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