Effective inhibition of porcine transmissible gastroenteritis virus replication in ST cells by shRNAs targeting RNA-dependent RNA polymerase gene

文献类型： 外文期刊

第一作者： Zhou, Jun-fang

作者： Zhou, Jun-fang;Hua, Xiu-guo;Cui, Li;Zhu, Jian-guo;Miao, De-nian;Zou, Yong;He, Xi-zhong;Su, Wan-guo

作者机构：

关键词： TGEV;RNA interference (RNAi);short hairpin RNA (shRNA);RdRp;ST

期刊名称：ANTIVIRAL RESEARCH （ 影响因子：5.97； 五年影响因子：5.801 ）

ISSN： 0166-3542

年卷期： 2007 年 74 卷 1 期

页码：

收录情况： SCI

摘要： Transmissible gastroenteritis virus (TGEV) is identified as one of the most important pathogenic agents during swine enteric infection, leading to high mortality in neonatal pigs and severe annual economic loss in swine-producing areas. Up to date, various vaccines developed against TGEV still need to be improved. To exploit the possibility of using RNA interference (RNAi) as a strategy against TGEV infection, two shRNA-expressing plasmids (pEGFP-U6/P1 and pEGFP-U6/P2) targeting the RNA-dependent RNA polymerase (RdRp) gene of TGEV were constructed and transfected into swine testicular (ST) cells. The cytopathic effect (CPE) and NITS assays demonstrated that both shRNAs were capable of protecting cells against TGEV invasion with very high specificity and efficiency. A real-time quantitative RT-PCR further confirmed that the amounts of viral RNAs in cell cultures pre-transfected with the two plasmids were reduced by 95.2% and up to 100%, respectively. Our results suggest that RNAi might be a promising new strategy against TGEV infection. (c) 2007 Elsevier B.V. All rights reserved.

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