A key structural gene, AaLDOX, is involved in anthocyanin biosynthesis in all red-fleshed kiwifruit (Actinidia arguta) based on transcriptome analysis
文献类型: 外文期刊
第一作者: Li, Yukuo
作者: Li, Yukuo;Fang, Jinbao;Qi, Xiujuan;Lin, Miaomiao;Zhong, Yunpeng;Sun, Leiming
作者机构:
关键词: Transcriptome;Kiwifruit;Anthocyanin;Structural gene;Differential expression
期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )
ISSN: 0378-1119
年卷期: 2018 年 648 卷
页码:
收录情况: SCI
摘要: Study on kiwifruit (Actinidia chinensis and A. deliciosa) color mainly concentrated in green and yellow-fleshed cultivars, less about molecular mechanism of red-fleshed trait formation, rarely in all red-fleshed fruit. Using 'Tianyuanhong' and 'Yongfengyihao' ('TY', a kind of all red-fleshed cultivar, from Actinidia arguta; 'YF', a kind of all green-fleshed cultivar, also from Actinidia arguta) as experimental material, we performed RNA-seq to obtain 202,742 unigenes with an average length of 603 bp and N50 of 873 bp via transcriptome data analysis. Of these unigenes, 72,508 (35.76%) were annotated and 997 were assigned to secondary metabolic pathways, of which 104 unigenes were involved in flavonoid and anthocyanin biosynthesis. According to the parameter log2fold-change and p-adjusted, 12 differentially expressed structural genes were selected for performing expression profiles and cluster analysis. Physiological traits including color ration, hue angle, and anthocyanin content were also investigated. From the results, we concluded AaLDOX (genes encoding leucoanthocyanidin dioxygenase) maybe the key gene controlling anthocyanin biosynthesis in flesh of 'TY' kiwifruit, which promoted accumulation of anthocyanin, finally leading to the red flesh coloration.
分类号:
- 相关文献
作者其他论文 更多>>
-
QTL mapping of fruit quality traits in tetraploid kiwiberry (Actinidia arguta)
作者:Wang, Ran;Li, Sikai;Lin, Miaomiao;Sun, Leiming;Gu, Hong;Li, Yukuo;Qi, Xiujuan;Fang, Jinbao;Wang, Ran;Wang, Ran;Bourke, Peter M.;Visser, Richard G. F.;Maliepaard, Chris
关键词:Kiwiberry; Polyploid genetics; QTL mapping; Breeding
-
RNA-Seq Analysis Reveals Potential Genes Involved in Plant Growth Regulator-Induced Ovary Development in Male Kiwifruit (Actinidia eriantha)
作者:Fu, Rong;Wei, Feng;Fu, Rong;Zhang, Min;Lin, Miaomiao;Fang, Jinbao;Wang, Ran;Li, Yukuo;Chen, Jinyong;Sun, Leiming;Qi, Xiujuan;Wei, Feng;Li, Yukuo;Qi, Xiujuan;Sun, Leiming
关键词:
Actinidia eriantha ; PGR; induction; ovary; RNA-Seq -
Chromosome-level genome assembly assisting for dissecting mechanism of anthocyanin regulation in kiwifruit (Actinidia arguta)
作者:Li, Yukuo;Song, Zhe;Zhan, Xu;Li, Xiaohan;Ye, Lingshuai;Lin, Miaomiao;Wang, Ran;Sun, Leiming;Gu, Hong;Chen, Jinyong;Fang, Jinbao;Qi, Xiujuan;Li, Yukuo;Lin, Miaomiao;Qi, Xiujuan;Song, Zhe;Li, Xiaohan;Zhan, Xu;Huang, Hailei;Guo, Jian
关键词:Genome assembly; Comparative genome; Gene expression; Anthocyanin biosynthesis;
Actinidia arguta -
Comparative transcriptome analysis and transient assays revealed AaGST and AaBGAL, respectively, contribute to skin and flesh coloration in A. arguta
作者:Zhan, Xu;Chen, Dixin;Zhan, Xu;Li, Yukuo;Song, Zhe;Li, Xiaohan;Ye, Lingshuai;Lin, Miaomiao;Wang, Ran;Sun, Leiming;Fang, Jinbao;Qi, Xiujuan;Li, Yukuo;Lin, Miaomiao;Qi, Xiujuan;Song, Zhe;Li, Xiaohan
关键词:Different tissues; Anthocyanin; Coloration; Actinidia arguta; Transcriptome
-
Construction of a high-density genetic map and QTL mapping of growth traits in kiwifruit
作者:Li, Sikai;Wang, Ran;Lin, Miaomiao;Gu, Hong;Li, Yukuo;Zhang, Min;Qi, Xiujuan;Lin, Miaomiao;Li, Yukuo;Qi, Xiujuan;Feng, Xin
关键词:Kiwifruit; Trunk diameter; Annual branch diameter; Single nucleotide polymorphism; Genetic map; QTL; Molecular marker
-
AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta
作者:Song, Zhe;Li, Xiaohan;Wei, Feng;Song, Zhe;Li, Yukuo;Zhan, Xu;Li, Xiaohan;Ye, Lingshuai;Lin, Miaomiao;Wang, Ran;Sun, Leiming;Chen, Jinyong;Fang, Jinbao;Qi, Xiujuan;Li, Yukuo;Lin, Miaomiao;Qi, Xiujuan;Zhan, Xu
关键词:
Actinidia arguta ; Anthocyanin biosynthesis; AaMYB61-like; AabHLH137; Transcription regulation -
The 14-3-3 gene AaGRF1 positively regulates cold tolerance in kiwifruit
作者:Zhang, Zhenzhen;Li, Sumei;Sun, Shihang;Zhang, Qina;Li, Yihang;Li, Yukuo;Liu, Mingyu;Li, Congcong;Sun, Leiming;Lin, Miaomiao;Qi, Xiujuan;Zhang, Zhenzhen;Sun, Shihang;Li, Yukuo;Lin, Miaomiao;Qi, Xiujuan;Li, Hongli
关键词:Kiwifruit; AaGRF1; Cold tolerance; RNA-seq