Relationship Between Protein Denaturation and Water Holding Capacity of Pork During Postmortem Ageing
文献类型: 外文期刊
第一作者: Li, Xia
作者: Li, Xia;Wei, Xiuli;Wang, Hang;Zhang, Chun-hui;Mehmood, Waris
作者机构:
关键词: Muscle;Meat;Aging;Protein structure;Myowater distribution
期刊名称:FOOD BIOPHYSICS ( 影响因子:3.114; 五年影响因子:3.407 )
ISSN: 1557-1858
年卷期: 2018 年 13 卷 1 期
页码:
收录情况: SCI
摘要: This study investigated water holding capacity (WHC), water distribution, and protein denaturation of pork loin chops (longissimus lumborum) packaged in polyethylene bags throughout display at 4 +/- 1 A degrees C for up to 1, 3, 5, 7 and 9 days. The drip loss of pork eventually increased following a decrease during the first 5 days of storage. Nuclear magnetic resonance (NMR) analysis revealed an increase in population of immobilized water P-22 from day 1 to day 5, meanwhile a sharp decrease after 9 days was noticed. However, an opposite trend was observed for the population of free water P-23. Correlation analysis indicated that myofibrillar protein solubility was negatively correlated with drip loss (p < 0.05), whereas sarcoplasmic protein measurement were not related to drip loss (p > 0.05). Furthermore, the content of alpha-helices increased during the first 5 days of storage (p < 0.05), which suggested increased WHC during the earlier period of postmortem storage. During the subsequent postmortem storage, the content of alpha-helices decreased significantly (p < 0.05), while the beta-sheets and beta-turns increased. The maximum temperatures (T-max) of three endothermic peaks were found to be 53.6 A degrees C, 65.2 A degrees C, and 77.6 A degrees C at 1 day postmortem. A significant decrease were observed for T-max peakI,T-max peakII,T-max peakIII at 9 d when compared to 1 d postmortem (p < 0.05), suggesting loss of thermal stability and protein denaturation.
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