A novel allele of TaGW2-A1 is located in a finely mapped QTL that increases grain weight but decreases grain number in wheat (Triticum aestivum L.)

文献类型： 外文期刊

第一作者： Zhai, Huijie

作者： Zhai, Huijie;Feng, Zhiyu;Song, Yane;Liu, Xinye;Qi, Zhongqi;Song, Long;Li, Jiang;Li, Linghong;Peng, Huiru;Hu, Zhaorong;Yao, Yingyin;Xin, Mingming;Sun, Qixin;Ni, Zhongfu;Zhai, Huijie;Feng, Zhiyu;Song, Yane;Liu, Xinye;Qi, Zhongqi;Song, Long;Li, Jiang;Li, Linghong;Peng, Huiru;Hu, Zhaorong;Yao, Yingyin;Xin, Mingming;Sun, Qixin;Ni, Zhongfu;Du, Xiaofen;Xiao, Shihe

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2018 年 131 卷 3 期

页码：

收录情况： SCI

摘要： A novel TaGW2-A1 allele was identified from a stable, robust QTL region, which is pleiotropic for thousand grain weight, grain number per spike, and grain morphometric parameters in wheat. Thousand grain weight (TGW) and grain number per spike (GNS) are two crucial determinants of wheat spike yield, and genetic dissection of their relationships can help to fine-tune these two components and maximize grain yield. By evaluating 191 recombinant inbred lines in 11 field trials, we identified five genomic regions on chromosomes 1B, 3A, 3B, 5B, or 7A that solely influenced either TGW or GNS, and a further region on chromosome 6A that concurrently affected TGW and GNS. The QTL of interest on chromosome 6A, which was flanked by wsnp_BE490604A_Ta_2_1 and wsnp_RFL_Contig1340_448996 and designated as QTgw/Gns.cau-6A, was finely mapped to a genetic interval shorter than 0.538 cM using near isogenic lines (NILs). The elite NILs of QTgw/Gns.cau-6A increased TGW by 8.33%, but decreased GNS by 3.05% in six field trials. Grain Weight 2 (TaGW2-A1), a well-characterized gene that negatively regulates TGW and grain width in wheat, was located within the finely mapped interval of QTgw/Gns.cau-6A. A novel and rare TaGW2-A1 allele with a 114-bp deletion in the 5' flanking region was identified in the parent with higher TGW, and it reduced TaGW2-A1 promoter activity and expression. In conclusion, these results expand our knowledge of the genetic and molecular basis of TGW-GNS trade-offs in wheat. The QTLs and the novel TaGW2-A1 allele are likely useful for the development of cultivars with higher TGW and/or higher GNS.

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