Highly sensitive detection of triazophos pesticide using a novel bio-bar-code amplification competitive immunoassay in a micro well plate-based platform
文献类型: 外文期刊
第一作者: Du, Pengfei
作者: Du, Pengfei;Jin, Maojun;Zhang, Chan;Chen, Ge;Cui, Xueyan;Zhang, Yudan;Zhang, Yanxin;Zou, Pan;Jiang, Zejun;Cao, Xiaolin;She, Yongxin;Jin, Fen;Wang, Jing
作者机构:
关键词: Triazophos;Gold nanoparticles;Magnetic microparticles;Bio-bar-code;Micro well plate
期刊名称:SENSORS AND ACTUATORS B-CHEMICAL ( 影响因子:7.46; 五年影响因子:6.743 )
ISSN: 0925-4005
年卷期: 2018 年 256 卷
页码:
收录情况: SCI
摘要: This study reports a novel bio-bar-code amplification competitive immunoassay for the detection of pesticide residue combined with a micro well plate. The sensing competitive immunoassay was made up of two types of particles: (1) the gold nanoparticles (AuNPs) with recognition elements (monoclonal antibody: McAb) and hundreds of thiolated single-strand oligonucleotide bar-codes; and (2) the magnetic microparticle (MMPs) with competition elements (hapten-OVA) for the target pesticide. Target pesticide and pesticide hapten competed with McAb labeled on the surface of AuNPs. The bar-code strands were released by dithiothreitol. Then a simple method based on micro well plate was used for the quantification of the bar-code strands. The bar-code strands with the half-complementary biotinylated DNA and half-complementary DNA-modified gold nanoparticles probe were immobilized onto the surface of a streptavidin-coated micro well plate, and the signal was amplified by silver enhancement. Triazophos (TAP), as a pesticide model, was detected by the proposed immunoassay. The linear range was from 2.5 x 10(-2) to 40.0 ng mL(-1), and LOD of the immunoassay was 1.96 x 10(-2) ng mL(-1). The correlation was further studied between this method and GC-MS analysis. Therefore, this method provided a promising approach for rapid detection and screening of pesticide residue. (C) 2017 Elsevier B.V. All rights reserved.
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