Fitness Advantage of mcr-1-Bearing IncI2 and IncX4 Plasmids in Vitro

文献类型： 外文期刊

第一作者： Wu, Renjie

作者： Wu, Renjie;Yi, Ling-xian;Yu, Lin-feng;Wang, Jing;Liu, Yiyun;Chen, Xiaojie;Lv, Luchao;Yang, Jun;Liu, Jian-Hua;Chen, Xiaojie

作者机构：

关键词： Escherichia coli;mcr-1;colistin;plasmid;fitness

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： The objective of this study was to assess the impact of diverse plasmids bearing colistin resistance gene mcr-1 on host fitness. Forty-seven commensal E. coli isolates recovered from the pig farm where mcr-1 was first identified were screened for mcr-1. mcr-1-bearing plasmids were characterized by sequencing. The fitness impact of mcr-1-bearing plasmids was evaluated by in vitro competition assays. Twenty-seven (57.5%) E. coli isolates were positive for mcr-1. The mcr-1 genes were mainly located on plasmids belonging to IncI2 (n = 5), IncX4 (n = 11), IncHI2/ST3 (n = 8), IncFII (n = 2), and IncY (n = 2). InHI2 plasmids also carried other resistance genes (floR, blaCTX-M, and fosA3) and were only detected in isolates from nursery pigs. Sequences of the representative mcr-1-bearing plasmids were almost identical to those of the corresponding plasmid types reported previously. An increase in the fitness of IncI2- and IncX4-carrying strains was observed, while the presence of IncHI2, IncFII and IncY plasmids showed a fitness cost although an insignificant fitness increase was initially observed in IncFII or IncY plasmids-containing strains. Acquisition of IncI2-type plasmid was more beneficial for host E. coli DH5 alpha than either IncHI2 or IncX4 plasmid, while transformants with IncHI2-type plasmid presented a competitive disadvantage against IncI2 or IncX4 plasmid containing strains. In conclusion, IncI2, IncX4, and IncHI2 were the major plasmid types driving the dissemination of mcr-1 in this farm. Increased fitness or co-selection by other antimicrobials might contribute to the further dissemination of the three epidemic mcr-1-positive plasmids (IncI2, IncX4, and IncHI2) in this farm and worldwide.

分类号：

  • 相关文献

[1]Molecular epidemiology of colistin-resistant Enterobacteriaceae in inpatient and avian isolates from China: high prevalence of mcr-negative Klebsiella pneumoniae. Wang, Xiaojuan,Wang, Ruobing,Jin, Longyang,Zhang, Yawei,Wang, Qi,Chen, Hongbin,Wang, Hui,Liu, Yuqing,Zhao, Min,Qi, Xiaomei,Qi, Xiaomei,Qi, Xiaomei. 2017

[2]Housefly (Musca domestica) and Blow Fly (Protophormia terraenovae) as Vectors of Bacteria Carrying Colistin Resistance Genes. Zhang, Jilei,Wang, Jiawei,Chen, Li,Yassin, Afrah Kamal,Li, Jing,Wang, Chengming,Yassin, Afrah Kamal,Kelly, Patrick,Butaye, Patrick,Butaye, Patrick,Gong, Jiansen,Cattley, Russell,Wang, Chengming,Qi, Kezong. 2018

[3]Identification and characterization of mcr mediated colistin resistance in extraintestinal Escherichia coli from poultry and livestock in China. Yassin, Afrah Kamal,Zhang, Jilei,Wang, Jiawei,Chen, Li,Lu, Guangwu,Li, Min,Wang, Yaoyao,Wang, Chengming,Yassin, Afrah Kamal,Kelly, Patrick,Butaye, Patrick,Butaye, Patrick,Gong, Jiansen,Wei, Lanjing,Qi, Kezong,Han, Xiangan,Price, Stuart,Hathcock, Terri,Wang, Chengming. 2017

[4]Characterization of NDM-5-positive extensively resistant Escherichia coli isolates from dairy cows. He, Tao,Wei, Ruicheng,Zhang, Lili,Sun, Lichang,Pang, Maoda,Wang, Ran,Wang, Yang.

[5]Generation of Newly Discovered Resistance Gene mcr-1 Knockout in Escherichia coli Using the CRISPR/Cas9 System. Sun, Lichang,He, Tao,Zhang, Lili,Pang, Maoda,Zhou, Yan,Bao, Hongduo,Wang, Ran,Zhang, Qiaoyan.

[6]pirAB(vp)-Bearing Vibrio parahaemolyticus and Vibrio campbellii Pathogens Isolated from the Same AHPND-Affected Pond Possess Highly Similar Pathogenic Plasmids. Dong, Xuan,Wang, Hailiang,Zou, Peizhuo,Xie, Guosi,Wan, Xiaoyuan,Yang, Qian,Zhu, Yanping,Chen, Mengmeng,Guo, Chengcheng,Wang, Wenchao,Huang, Jie,Bi, Dexi,Zou, Peizhuo,Chen, Mengmeng,Liu, Zhen. 2017

[7]Multidrug resistance genes in staphylococci from animals that confer resistance to critically and highly important antimicrobial agents in human medicine. Wendlandt, Sarah,Kadlec, Kristina,Fessler, Andrea T.,Schwarz, Stefan,Shen, Jianzhong,Wang, Yang,Wu, Congming,Li, Beibei,Zhang, Wan-Jiang.

[8]A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis. Peng, Ri-He,Xiong, Ai-Sheng,Yao, Quan-Hong.

[9]GFP as a marker for transient gene transfer and expression in Mycoplasma hyorhinis. Ishag, Hassan Z. A.,Liu, Maojun,Yang, Ruosong,Xiong, Qiyan,Feng, Zhixin,Shao, Guoqing,Ishag, Hassan Z. A.. 2016

[10]Comparative study of the immune effect of an Edwardsiella tarda antigen in two forms: Subunit vaccine vs DNA vaccine. Sun, Yun,Sun, Li,Sun, Yun,Liu, Chun-Sheng.

[11]The technology of large-scale pharmaceutical plasmid purification by cetyltrimethylammonium bromide and Tritonx-114. Hou, S. H.,Zhu, H. F.,Zhang, Q.,Yuan, W. F.,Sun, H. C.. 2010

[12]Nickel-resistant determinant from Leptospirillum ferriphilum. Tian, Jian,Wu, Ningfeng,Li, Jiang,Liu, Yajie,Guo, Jun,Yao, Bin,Fan, Yunliu.

[13]Multiplex event-specific qualitative polymerase chain reaction for detecting three transgenic rice lines and application of a standard plasmid as a quantitative reference molecule. Wang, Xiaofu,Shen, Wenbiao,Wang, Xiaofu,Chen, Xiaoyun,Xu, Junfeng,Wang, Pengfei.

[14]Inducible expression of calreticulin-N58 in Pichia pastoris by high density cell culture. Su, D. X.,Zhang, A. L.,Liu, Z. W.,Luo, J. X.,Zhang, T. Y.,Luo, J. X.,Zhang, T. Y.,Yi, G. H.,Rao, L. Y.,Zhou, Z. J..

[15]Parallel assembly for multiple site-directed mutagenesis of plasmids. Yan, Pu,Shen, Wentao,Zhou, Peng,Duan, Jun,Gao, XinZheng.

[16]Ambient insect pressure and recipient genotypes determine fecundity of transgenic crop-weed rice hybrid progeny: Implications for environmental biosafety assessment. Xia, Hui,Zhang, Hongbin,Wang, Wei,Yang, Xiao,Xia, Hanbing,Xu, Kai,Cai, Xingxing,Lu, Bao-Rong,Xia, Hui,Wang, Feng,Su, Jun. 2016

[17]Analysis of Fitness Predominance for Gaoyou Duck's Double Yolk Egg. Zhang, Tangjie,Chang, Hong,Li, Hui-Fang,Chen, Kuanwei,Zhao, Yonggao,Xuec, Minkai,Zhang, Shengfu. 2011

[18]Effects of SRBSDV-infected rice plants on the fitness of vector and non-vector rice planthoppers. Xu, Hong-Xing,Zheng, Xu-Song,Yang, Ya-Jun,Zhang, Jue-feng,Lu, Zhong-Xian,He, Xiao-Chan. 2016

[19]Effects of insect-resistance transgenes on fecundity in rice (Oryza sativa, Poaceae): A test for underlying costs. Chen, LY,Snow, AA,Wang, F,Lu, BR. 2006

[20]Characterization of surface antigen protein 1 (SurA1) from Acinetobacter baumannii and its role in virulence and fitness. Liu, Dong,Liu, Zeng-Shan,Hu, Pan,Li, Yan-Song,Lu, Shi-Ying,Liu, Nan-Nan,Ma, Xiao-Long,Chi, Dan,Chang, Jiang,Shui, Yi-Ming,Li, Zhao-Hui,Ahmad, Waqas,Zhou, Yu,Ren, Hong-Lin,Fu, Bao-Quan,Cai, Ling.

作者其他论文 更多>>

微信小程序