Generation and application of replication-competent Venus-expressing H5N1, H7N9, and H9N2 influenza A viruses

文献类型： 外文期刊

第一作者： Wang, Guangwen

作者： Wang, Guangwen;Zhang, Jie;Kong, Fandi;Li, Qibing;Wang, Jinliang;Ma, Shujie;Zhao, Yuhui;Liang, Libin;Li, Junping;Sun, Nan;Guan, Lizheng;Zhou, Yuan;Zhou, Chenchen;Huang, Shanyu;Bu, Zhigao;Jiang, Li;Chen, Hualan;Li, Chengjun

作者机构：

关键词： Replication-competent;Influenza A virus;H5N1;H7N9;H9N2;Venus

期刊名称：SCIENCE BULLETIN （ 影响因子：11.78； 五年影响因子：7.658 ）

ISSN： 2095-9273

年卷期： 2018 年 63 卷 3 期

页码：

收录情况： SCI

摘要： The generation and application of replication-competent influenza A virus (IAV) expressing a reporter gene represent a valuable tool to elucidate the mechanism of viral pathogenesis and establish new countermeasures to combat the threat of influenza. Here, replication-competent IAVs with a neuraminidase (NA) segment harboring a fluorescent reporter protein, Venus, were generated in the background of H5N1, H7N9, and H9N2 influenza viruses, the three subtypes of viruses with imminent pandemic potential. All three reporter viruses maintained virion morphology, replicated with similar or slightly reduced titers relative to their parental viruses, and stably expressed the fluorescent signal for at least two passages in embryonated chicken eggs. As a proof of concept, we demonstrated that these reporter viruses, used in combination with a high-content imaging system, can serve as a convenient and rapid tool for the screening of antivirals and host factors involved in the virus life cycle. Moreover, the reporter viruses demonstrated similar growth properties and tissue tropism as their parental viruses in mice, among which the H7N9 NA-Venus virus could potentially be used in ex vivo studies to better understand H7N9 pathogenesis or to develop novel therapeutics. (C) 2018 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.

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