Optimizing the nested PCR method for Decapod iridescent virus 1 (DIV1) targeting ATPase gene by reselecting the inner primers
文献类型： 外文期刊
第一作者： Xing, Jing-Yi
作者： Xing, Jing-Yi;Guo, Xiao-Meng;Wang, Meng;Guan, Xin;Qiu, Liang;Zhang, Qing-Li;Huang, Jie;Qiu, Liang;Zhang, Qing-Li;Huang, Jie;Xing, Jing-Yi;Li, An-Qi;Zhang, Qing-Li;Guo, Xiao-Meng;Zhang, Qing-Li;Huang, Jie;Wang, Meng;Guan, Xin;Qiu, Liang;Zhang, Qing-Li
作者机构：
关键词： Decapod iridescent virus 1; Shrimp hemocyte iridescent virus; Cherax quadricarinatus iridovirus; Nested PCR; ATPase
期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.4； 五年影响因子：3.0 ）
ISSN： 0022-2011
年卷期： 2024 年 207 卷
页码：
收录情况： SCI
摘要： DIV1 has the characteristics of fast transmission and a broad host range. Its infection leads to a high mortality rate, posing a serious threat to the global crustacean aquaculture industry. In order to increase the accuracy of DIV1 detection and reduce the difficulty of result interpretation, this study modified the original nested PCR method targeting the DIV1 ATPase gene. The internal primers for the nested PCR were redesigned to produce a 338 bp amplification product in the second step PCR, effectively distinguishing the target band from primer dimers. The newly established nested PCR method exhibits strong specificity and high sensitivity, with a detection limit as low as 1.37 x 101 copies/reaction. The developed nested PCR assay provides new technical support for the accurate detection of DIV1 in global crustacean aquaculture.
分类号：
- 相关文献
作者其他论文 更多>>
-
Chemotaxis of rhizosphere Pseudomonas sp. induced by foliar spraying of lanthanum reduces cadmium uptake by pakchoi
作者：Wang, Meng;Yu, Lei;Wang, Jing;Qin, Luyao;Sun, Xiaoyi;Liu, Jiaxiao;Han, Yun;Chen, Shibao
关键词：Cadmium toxicity; Plant growth; Root exudates; Pseudomonas; Chemotaxis
-
Biodegradable film drip fertigation is more conducive to reducing the diversity and abundance of antibiotic resistance genes than plastic film drip fertigation
作者：Li, Yingjie;Yuan, Yuhan;Li, Cuilan;Zhang, Jinjing;Liu, Hang;Wang, Meng;Li, Qian;Zhang, Lei;Sun, Bo;Qin, Yubo
关键词：Antibiotic resistance genes; Biodegradable film drip fertigation; Bacterial diversity; HT-PCR
-
Changes in the muscle quality of crayfish (Procambarus clarkii) under low-temperature storage
作者：Li, Hailan;Qiu, Liang;Liao, Tao;Cai, Fang;Zu, Xiaoyan;Li, Hailan;Liu, Qi;Qiu, Liang;Liao, Tao;Zu, Xiaoyan;Fu, Jianlong;Huang, Yongbing;Cai, Fang
关键词：Procambarus clarkii; Low temperature storage; Muscle quality; Volatile components; Fatty acid composition
-
Long short-term search session-based document re-ranking model
作者：Liu, Jianping;Wang, Meng;Wang, Yingfei;Chu, Xintao;Liu, Jianping;Wang, Jian
关键词：Document re-ranking; Long short-term session search; Memory network; BERT; User intent
-
Effects of the skp1 gene of the SCF complex on lipid metabolism and response to abiotic stress in Chlamydomonas reinhardtii
作者：Deng, Xiao Dong;Fei, Xiao Wen;Deng, Xiao Dong;Wang, Meng;Liu, Si Hang;Xu, Dian Long;Deng, Xiao Dong;Wang, Meng;Liu, Si Hang;Xu, Dian Long;Deng, Xiao Dong;Deng, Xiao Dong
关键词：SCF(SKP1-Cullin1-F-box) complex; Skp1; E3 ubiquitin ligase; Chlamydomonas reinhardtii; lipid metabolism; abiotic stress
-
Magnetic Co/Fe bimetallic confined nitrogen dopped porous carbon to activate peroxymonosulfate for efficient degradation of mycotoxins: Radical and non-radical pathways
作者：Wang, Yudan;Li, Yu;Zhai, Wenlei;Fang, Lin;Wang, Meng
关键词：Co/Fe@N-PC; Mycotoxins; Peroxymonosulfate; DFT calculations; Toxicity evaluation
-
A core-shell AuNRs@BUT-16 nanocomposite for enhancement SERS detection and efficient removal of deoxynivalenol
作者：Tao, Jing;Wang, Yudan;Zhai, Wenlei;Wang, Meng
关键词：Deoxynivalenol; Metal-organic frameworks; AuNRs; SERS; Molecular docking
