Overexpression of the maize ZmAMT1;1a gene enhances root ammonium uptake efficiency under low ammonium nutrition

文献类型： 外文期刊

第一作者： Zhao, Yang

作者： Zhao, Yang;Liu, Zhi;Duan, Fengying;An, Xia;Gu, Riliang;Wang, Zhangkui;Chen, Fanjun;Yuan, Lixing;Duan, Fengying;Liu, Xiangguo;Hao, Dongyun

作者机构：

关键词： AMT;Ammonium uptake;Nitrogen use efficiency;Transgenic plants;Post-translational regulation;Zea mays

期刊名称：PLANT BIOTECHNOLOGY REPORTS （ 影响因子：2.01； 五年影响因子：1.907 ）

ISSN： 1863-5466

年卷期： 2018 年 12 卷 1 期

页码：

收录情况： SCI

摘要： High-affinity ammonium uptake in maize roots is mainly mediated by AMT1-type ammonium transporters ZmAMT1;1a and ZmAMT1;3, but whether the increased expression of ZmAMTs genes is able to enhance ammonium uptake capacity and subsequently improves overall nitrogen use efficiency remains to be elucidated. In this work, ZmAMT1;1a-overexpression transgenic maize plants were generated with the elevated levels of transcripts and proteins, and phenotypically analyzed together with wild-type plants grown in nutrient solution under two regimes of ammonium supply. Under low ammonium nutrition (0.04 mM), in relative to wild-type plants, the maize transgenic lines showed an approximately 17% increases in the high-affinity ammonium uptake capacity of roots as revealed by N-15-labeled ammonium influx assay and further contributed to about 7% increases in the total nitrogen uptake at the whole plant level. By contrast, when ammonium was supplied in high amounts (1 mM), wild-type plants expressed higher levels of ZmAMT1;1a, but exhibited a lower ammonium uptake capacity in roots. Furthermore, the transgenic maize line accumulated more amounts of ZmAMT1;1a protein, but did not translate into an enhanced ammonium acquisition, suggesting a possible post-translational down-regulation of ZmAMT1;1a by high ammonium. This study proved the possibility to enhance ammonium acquisition by elevating ZmAMTs expression in maize roots and provided an effective transgenic approach on developing high nitrogen use efficient maize cultivars.

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