Liver epithelioid progenitor cells derived from fetal Luxi bovine alleviate liver fibrosis

文献类型： 外文期刊

第一作者： Wang, Kunfu

作者： Wang, Kunfu;Zheng, Dong;Wang, Kunfu;Liu, Hao;Yang, Jinjuan;Ma, Caiyun;Zhang, Zebiao;Guan, Weijun;Liu, Hao

作者机构：

关键词： Hepatocyte differentiation;Liver epithelioid progenitor cells;Stem cells therapy;Liver fibrosis;Heterogeneous cell transplantation

期刊名称：CYTOTECHNOLOGY （ 影响因子：2.058； 五年影响因子：2.362 ）

ISSN： 0920-9069

年卷期： 2018 年 70 卷 1 期

页码：

收录情况： SCI

摘要： Liver epithelioid progenitor cells (LEPCs) have important roles in liver therapy because of their hepatic differentiation potency in vitro and in vivo. Despite many researches on humans, mice, and rats, equivalent progenitor cells derived from bovine are relatively rare. The purpose of our current study is to characterize bovine LEPCs, and research on the cure potency of this heteroplastic progenitor cells on mice liver fibrosis. We have used collagenase IV digesting and differential adhesion method to isolate slabstone shape, EpCAM, LGR5, NCAM1 and SOX9 positive progenitor cells from fetal Luxi bovine liver. When cultured in hepatic differentiation media containing 20 ng/ml Oncostatin M, LEPCs can differentiate into hepatocytes in vitro. After 4 weeks of intravenous tail vein injection into CCl4-injured mouse liver, LEPCs engrafted into liver parenchyma, differentiated into ALB positive hepatocytes, and could alleviate liver fibrosis through down regulating fibrosis genes-Tgfb1 and alpha-SMA as well as decreasing expression of collagen gene Col1a1, Col3a1, and Col4a1, and regain liver function by recovering ALT and AST. Our findings provided a useful tool for studying liver development in vitro, new cell resource for heterograft on mouse liver diseases, and a new platform for researches on immune rejection of heterogeneous cell transplantation.

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