Comparative genetic analysis and pathological characteristics of goose parvovirus isolated in Heilongjiang, China

文献类型： 外文期刊

第一作者： Niu, Yinjie

作者： Niu, Yinjie;Zhao, Lili;Liu, Baihan;Yin, Haichang;Huo, Hong;Chen, Hongyan;Liu, Baihan;Liu, Jingli;Yang, Fan

作者机构：

关键词： Goose parvovirus;Phylogenetic analysis;Pathological changes

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2018 年 15 卷

页码：

收录情况： SCI

摘要： Background: Goose parvovirus (GPV) causes acute enteritis, hepatitis, myocarditis and high morbidity and mortality in geese and ducks. GPV H strain was isolated from a Heilongjiang goose farm where the geese were showing signs of hemorrhage in the brain, liver, and intestinal tract. In this study, we explored the genetic diversity among waterfowl parvovirus isolates and the pathological characteristics of GPV H in Shaoxing ducklings. Methods: The complete capsid protein (VP) and non-structural (NS) sequences of the isolated H strain were sequenced, and phylogenetic trees of VP and NS were constructed in MEGA version 5.05 using the neighborjoining method. Three-day-old Shaoxing ducklings were inoculated with GPV and were euthanized at 1, 2, 4, 6, and 8 days post-inoculation (PI), and their organs were removed and collected. The organs of 6-day PI ducklings were fixed in formalin, embedded in paraffin, sectioned for histology, stained with HE and analyzed for pathological lesions. The distribution of the GPV H strain in the tissues of the inoculated ducklings was detected using the polymerase chain reaction (PCR) method. Results: Genetic analysis of the NS and VP genes indicated that the H strain was closely related to strains circulating in China during 1999-2014, and the nucleic acid identity of those strains was 98%-99%. Classical symptoms were observed in the inoculated ducklings. GPV remained in many tissues and replicated in a majority of the tissues, leading to histopathological lesions in four tissues. Conclusions: We first reported the distribution and histopathological lesions of a Chinese strain of GPV in infected shaoxing ducklings. This H strain was moderate pathogenic for Shaoxing ducklings.

分类号：

  • 相关文献

[1]Phylogenetic analysis of VP1 gene sequences of waterfowl parvoviruses from the Mainland of China revealed genetic diversity and recombination. Wang, Shao,Cheng, Xiao-xia,Chen, Shao-ying,Lin, Feng-qiang,Chen, Shi-long,Zhu, Xiao-li,Wang, Jin-xiang,Huang, Mei-qing,Zheng, Min,Wang, Shao,Cheng, Xiao-xia,Chen, Shao-ying,Lin, Feng-qiang,Chen, Shi-long,Zhu, Xiao-li,Wang, Jin-xiang,Huang, Mei-qing,Zheng, Min.

[2]Identification of a recombinant Muscovy Duck parvovirus (MDPV) in Shanghai, China. Zhu, Yumin,Zhou, Zongqing,Huang, Yu,Yu, Ruisong,Dong, Shijuan,Li, Zhen,Zhu, Yumin,Zhou, Zongqing,Huang, Yu,Yu, Ruisong,Dong, Shijuan,Li, Zhen,Zhang, Yuanshu.

[3]Novel duck parvovirus identified in Cherry Valley ducks (Anas platyrhynchos domesticus), China. Li, Chuanfeng,Li, Qi,Chen, Zongyan,Liu, Guangqing.

[4]Genetic Characterization of a Potentially Novel Goose Parvovirus Circulating in Muscovy Duck Flocks in Fujian Province, China. Wang, Shao,Cheng, Xiao-Xia,Chen, Shao-Ying,Zhu, Xiao-Li,Chen, Shi-Long,Lin, Feng-Qiang,Li, Zhao-Long,Wang, Shao,Cheng, Xiao-Xia,Chen, Shao-Ying,Chen, Shi-Long,Lin, Feng-Qiang. 2013

[5]Rapid diagnosis of goose viral infections by multiplex PCR. Chen, Zongyan,Li, Chuanfeng,Li, Guoxin,Yu, Hai,Jiang, Yifeng,Yan, Liping,Meng, Chunchun,Zhou, Yanjun,Tong, Guangzhi,Liu, Guangqing.

[6]Complete Genome Sequence of Goose Parvovirus Isolated from Anser cygnoides in China. Wan, Chun-he,Hu, Kai-hui,Wan, Chun-he,Huang, Yu,Shi, Shao-hua,Fu, Guang-hua,Cheng, Long-fei,Chen, Hong-mei,Fu, Qiu-ling,Chen, Cui-teng. 2016

[7]Evidence for natural recombination in the capsid gene VP2 of Taiwanese goose parvovirus. Wang, Shao,Cheng, Xiaoxia,Chen, Shaoying,Lin, Fengqiang,Chen, Shilong,Zhu, Xiaoli,Wang, Jingxiang,Wang, Shao,Cheng, Xiaoxia,Chen, Shaoying,Lin, Fengqiang,Chen, Shilong,Zhu, Xiaoli,Wang, Jingxiang.

[8]Expression, Purification, Characterization and Subcellular Localization of the Goose Parvovirus Rep1 Protein. Chen, Zongyan,Li, Chuanfeng,Peng, Gaojing,Liu, Guangqing. 2013

[9]Immunogenicity of virus-like particles containing modified goose parvovirus VP2 protein. Chen, Zongyan,Li, Chuanfeng,Zhu, Yingqi,Wang, Binbin,Meng, Chunchun,Liu, Guangqing.

[10]Cloning of the genome of a goose parvovirus vaccine strain SYG61v and rescue of infectious virions from recombinant plasmid in embryonated goose eggs. Wang, Jianye,Duan, Jinkun,Meng, Xia,Jiang, Zhiwei,Zhu, Guoqiang,Wang, Jianye,Duan, Jinkun,Meng, Xia,Jiang, Zhiwei,Zhu, Guoqiang,Gong, Jiansen. 2014

[11]Generation and evaluation of a recombinant genotype VII Newcastle disease virus expressing VP3 protein of Goose parvovirus as a bivalent vaccine in goslings. Wang, Jianzhong,Cong, Yanlong,Yin, Renfu,Ding, Zhuang,Wang, Jianzhong,Cong, Yanlong,Yin, Renfu,Ding, Zhuang,Wang, Chunfeng,Feng, Na,Yang, Songtao,Xia, Xianzhu,Xiao, Yueqiang,Wang, Wenxiu,Liu, Xiufan,Hu, Shunlin,Ding, Chan,Yu, Shengqing.

[12]Development and evaluation of a VP3-ELISA for the detection of goose and Muscovy duck parvovirus antibodies. Zhang, Yun,Liu, Ming,Zhang, Dabing,Li, Gang,Tong, Guangzhi. 2010

[13]Genomic Characterization of Goose Parvovirus and Muscovy Duck Parvovirus Co-infection in Fujian, China. Wan, Chun-he,Hu, Kai-hui,Wan, Chun-he,Chen, Hong-mei,Fu, Qiu-ling,Fu, Guang-hua,Cheng, Long-fei,Shi, Shao-hua,Huang, Yu. 2015

[14]Identification of target cells for Goose parvovirus infection in the immune system organs. Zhu, L. Q.,Tao, J.,Wang, J. Y.,Zhang, X. J.,Wang, X. B.,Zhu, G. Q.,Ding, X. Y.,Ding, X. Y.. 2010

[15]Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses. Wan, Chun-He,Chen, Hong-Mei,Fu, Qiu-Ling,Shi, Shao-Hua,Fu, Guang-Hua,Cheng, Long-Fei,Chen, Cui-Teng,Huang, Yu,Hu, Kai-Hui.

[16]Molecular Characterization and Phylogenetic Analysis of New Variants of the Porcine Epidemic Diarrhea Virus in Gansu, China in 2012. Tian, Yufei,Liu, Yuxiu,Yang, Zhenguo,Tian, Yufei,Yu, Zhijun,Huang, Jing,Xin, Yue,Li, Yuanguo,Wang, Tiecheng,Huang, Geng,Feng, Na,Yang, Songtao,Gao, Yuwei,Xia, Xianzhu,Yu, Zhijun,Fan, Shengtao,Yu, Zhijun,Fan, Shengtao,Cheng, Kaihui. 2013

[17]Biotype status and distribution of Bemisia tabaci (Hemiptera : Aleyrodidae) in shandong province of China based on mitochondrial DNA markers. Chu, D.,Jiang, T.,Liu, G. X.,Jiang, D. F.,Tao, Y. L.,Fan, Z. X.,Zhou, H. X.,Bi, Y. P.. 2007

[18]The introduction of the exotic Q biotype of Bemisia tabaci from the Mediterranean region into China on ornamental crops. Chu, D,Zhang, YJ,Brown, JK,Cong, B,Xu, BY,Wu, QJ,Zhu, GR. 2006

[19]Characterization of complete mitochondrial genome of Dezhou donkey (Equus asinus) and evolutionary analysis. Sun, Yan,Jiang, Qiang,Yang, Chunhong,Wang, Xiuge,Ju, Zhihua,Huang, Jinming,Zhong, Jifeng,Wang, Changfa,Tian, Fang,Wang, Yinchao,Zhou, Xiangshan,Ma, Yong.

[20]Genome-wide identification and characterization of SnRK2 gene family in cotton (Gossypium hirsutum L.). Zhao Liu,Xiaoyang Ge,Zuoren Yang,Li, Fuguang,Zhang, Xueyan,Chaojun Zhang,Ge Zhao,Eryong Chen,Ji Liu,Xueyan Zhang,Fuguang Li. 2017

作者其他论文 更多>>

微信小程序