Altered Serum Metabolite Profiling and Relevant Pathway Analysis in Rats Stimulated by Honeybee Venom: New Insight into Allergy to Honeybee Venom
文献类型: 外文期刊
第一作者: Zhao, Yazhou
作者: Zhao, Yazhou;Li, Zhiguo;Nie, Hongyi;Su, Songkun;Zhao, Yazhou;Zhang, Jianmei;Peng, Wenjun;Chen, Yanping
作者机构:
关键词: honeybee venom stimulation;serum metabonomics;metabolite profiling;H-1 NMR
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )
ISSN: 0021-8561
年卷期: 2018 年 66 卷 4 期
页码:
收录情况: SCI
摘要: To improve our understanding of the disturbed metabolic pathways and cellular responses triggered by honeybee venom stimulation, we compared the changes in serum metabolites in rats, either stimulated or not by honeybee venom, by performing H-1 nuclear magnetic resonance (NMR) spectrometry-based metabonomics to identify potential biomarkers. In this study, 65 metabolites were structurally confirmed and quantified and the following results were obtained. First, by pattern recognition analysis, 14 metabolites were selected as potential biomarkers 3 h after venom stimulation. Second, metabolic pathway analysis showed that methane metabolism, glyoxylate and dicarboxylate metabolism, tricarboxylic acid cycle, glycine, serine, and threonine metabolism, arginine and proline metabolism were affected. Finally, the time-dependent metabolic modifications indicated that rats could recover without medical treatment 24 h after venom stimulation. In summary, this new insight into the changes in serum metabolites in rats after honeybee venom stimulation has enhanced our understanding of the response of an organism to honeybee venom.
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