Genome and secretome analysis of Pochonia chlamydosporia provide new insight into egg-parasitic mechanisms

文献类型： 外文期刊

第一作者： Lin, Runmao

作者： Lin, Runmao;Qin, Feifei;Zhang, Xi;Lu, Jun;Gao, Yaoyao;Cheng, Xinyue;Lin, Runmao;Qin, Feifei;Shen, Baoming;Shi, Qianqian;Liu, Chichuan;Zhang, Xi;Wang, Gang;Mao, Zhenchuan;Ling, Jian;Yang, Yuhong;Xie, Bingyan;Suarez-Fernandez, Marta;Lopez-Moya, Federico;Vicente Lopez-Llorca, Luis;Cheng, Xinyue;Xie, Bingyan

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2018 年 8 卷

页码：

收录情况： SCI

摘要： Pochonia chlamydosporia infects eggs and females of economically important plant-parasitic nematodes. The fungal isolates parasitizing different nematodes are genetically distinct. To understand their intraspecific genetic differentiation, parasitic mechanisms, and adaptive evolution, we assembled seven putative chromosomes of P. chlamydosporia strain 170 isolated from root-knot nematode eggs (similar to 44 Mb, including 7.19% of transposable elements) and compared them with the genome of the strain 123 (similar to 41 Mb) isolated from cereal cyst nematode. We focus on secretomes of the fungus, which play important roles in pathogenicity and fungus-host/environment interactions, and identified 1,750 secreted proteins, with a high proportion of carboxypeptidases, subtilisins, and chitinases. We analyzed the phylogenies of these genes and predicted new pathogenic molecules. By comparative transcriptome analysis, we found that secreted proteins involved in responses to nutrient stress are mainly comprised of proteases and glycoside hydrolases. Moreover, 32 secreted proteins undergoing positive selection and 71 duplicated gene pairs encoding secreted proteins are identified. Two duplicated pairs encoding secreted glycosyl hydrolases (GH30), which may be related to fungal endophytic process and lost in many insect-pathogenic fungi but exist in nematophagous fungi, are putatively acquired from bacteria by horizontal gene transfer. The results help understanding genetic origins and evolution of parasitism-related genes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序