Molecular characterization of Babesia microti thioredoxin (BmTrx2) and its expression patterns induced by antiprotozoal drugs

文献类型： 外文期刊

第一作者： Huang, Jingwei

作者： Huang, Jingwei;Xiong, Kang;Zhang, Houshuang;Zhao, Yanzhen;Cao, Jie;Gong, Haiyan;Zhou, Yongzhi;Zhou, Jinlin

作者机构：

关键词： Babesia microti;Thioredoxin 2;Antioxidant;Drug response

期刊名称：PARASITES & VECTORS （ 影响因子：3.876； 五年影响因子：3.959 ）

ISSN： 1756-3305

年卷期： 2018 年 11 卷

页码：

收录情况： SCI

摘要： Background: Human babesiosis is an infectious disease that is epidemic in various regions all over the world. The predominant causative pathogen of this disease is the intra-erythrocytic parasite Babesia microti. The thioredoxin system is one of the major weapons that is used in the resistance to the reactive oxygen species (ROS) and reactive nitrogen species (RNS) produced by host immune system. In other intra-erythrocytic apicomplexans like the malaria parasite Plasmodium falciparum, anti-oxidative proteins are promising targets for the development of anti-parasitic drugs. However, to date, the sequences and biological properties of thioredoxins and thioredoxin-like molecules of B. microti remain unknown. Understanding the molecular characterization and function of B. microti thioredoxins may help to develop anti-Babesia drugs and controlling babesiosis. Methods: The full-length B. microti thioredoxin 2 (BmTrx2) gene was obtained using a rapid amplification of cDNA ends (RACE) method, and the deduced BmTrx2 amino acid sequence was analyzed using regular bioinformatics tools. Recombinant BmTrx2 protein was expressed in vitro and purified using His-tag protein affinity chromatography resins. Reverse transcription PCR, quantitative real-time PCR and Western blot were employed to detect the expression and native proteins of BmTrx2. Indirect immunofluorescence assay was used to localize BmTrx2 in B. microti. Bovine insulin reduction assays were used to determine the enzyme activity of the purified recombinant BmTrx2 protein. Results: The full-length BmTrx2 was 564 bp with a 408 bp open reading frame encoding a protein of 135 amino acids. The predicted molecular weight of the protein was 15.5 kDa. A conserved thioredoxin-like family domain was found in BmTrx2. The expression of BmTrx2 was upregulated on both the third and eighth day post-infection in mice, whereas expression was downregulated during the beginning and later stages. The results of Western blot analysis showed the native BmTrx2 in parasite lysates could be detected by mouse anti-BmTrx2 serum and that the recombinant BmTrx2 protein could be recognized by serum of B. microti-infected mice. Immunofluorescence microscopy showed that BmTrx2 localized in the cell cytoplasm of B. microti merozoites in B. microti-infected red blood cells. The results of bovine insulin reduction assay indicated the purified recombinant BmTrx2 protein possesses antioxidant enzyme activity. Dihydroartemisinin and quinine, known anti-malaria drugs, and clindamycin, a known anti-babesiosis drug, induced significantly higher upregulation of BmTrx2 mRNA. Conclusions: Our results indicate that BmTrx2 is a functional enzyme with antioxidant activity and may be involved in the response of B. microti to anti-parasite drugs.

分类号：

  • 相关文献

[1]Babesia microti Aldo-keto Reductase-Like Protein Involved in Antioxidant and Anti-parasite Response. Huang, Qiang,Cao, Jie,Zhou, Yongzhi,Huang, Jingwei,Gong, Haiyan,Zhang, Houshuang,Zhou, Jinlin,Zhu, Xing-Quan,Zhu, Xing-Quan,Zhou, Jinlin. 2017

[2]Expression of truncated Babesia microti apical membrane protein 1 and rhoptry neck protein 2 and evaluation of their protective efficacy. Wang, Guanbo,Efstratiou, Artemis,Moumouni, Paul Franck Adjou,Liu, Mingming,Jirapattharasate, Charoonluk,Guo, Huanping,Gao, Yang,Suzuki, Hiroshi,Igarashi, Ikuo,Xuan, Xuenan,Cao, Shinuo,Zhou, Mo.

[3]Identification and characterization of an interspersed repeat antigen of Babesia microti (BmIRA). Cao, Shinuo,Luo, Yuzi,Aboge, Gabriel Oluga,Terkawi, Mohamad Alaa,Masatani, Tatsunori,Suzuki, Hiroshi,Igarashi, Ikuo,Nishikawa, Yoshifumi,Xuan, Xuenan,Luo, Yuzi.

[4]Identification of 2-Cys Peroxiredoxin (BmTPx-2) as Antioxidant Active Molecule from Babesia microti. Hai, Xunan,Zhang, Houshuang,Wang, Zhonghua,Gong, Haiyan,Cao, Jie,Zhou, Yongzhi,Zhou, Jinlin,Zhou, Jinlin. 2017

[5]Identification of a thioredoxin reductase from Babesia microti during mammalian infection. Zhao, Shaoruo,Gong, Haiyan,Zhou, Yongzhi,Zhang, Houshuang,Cao, Jie,Zhou, Jinlin,Zhou, Jinlin.

[6]Evaluation on Infectivity of Babesia microti to Domestic Animals and Ticks Outside the Ixodes Genus. Wu, Jiajun,Cao, Jie,Zhou, Yongzhi,Zhang, Houshuang,Gong, Haiyan,Zhou, Jinlin,Zhou, Jinlin. 2017

[7]Identification and functional study of a novel 2-cys peroxiredoxin (BmTPx-1) of Babesia microti. Zhang, Houshuang,Wang, Zhonghua,Gong, Haiyan,Cao, Jie,Zhou, Yongzhi,Zhou, Jinlin,Zhou, Jinlin.

[8]Effect of irradiation and storage time on lipid oxidation of chilled pork. Cheng, Anwei,Xu, Tongcheng,Du, Fangling,Wang, Wenliang,Zhu, Qingjun,Wan, Fachun. 2011

[9]Antioxidant and tyrosinase inhibitory activity of Rosa roxburghii fruit and identification of main bioactive phytochemicals by UPLC-Triple-TOF/MS. Zeng, Fangfang,Limwachiranon, Jarukitt,Li, Li,Feng, Simin,Luo, Zisheng,Ge, Zhiwei,Wang, Yansheng.

[10]Antioxidative and Hepatoprotective Activities of Deinoxanthin-Rich Extract from Deinococcus radiodurans R1 against Carbon Tetrachloride-Induced Liver Injury in Mice. Cheng, Jianhui,Zheng, Zhiguo,Tian, Bing,Hua, Yuejin,Cheng, Jianhui,Zhang, Zuofa,Lv, Guoying,Zheng, Zhiguo. 2014

[11]Physiological Mechanism of Enhancing Salt Stress Tolerance of Perennial Ryegrass by 24-Epibrassinolide. Wu, Wenli,Zhang, Qiang,Yang, Zhiping,Ervin, Erik. H.,Zhang, Xunzhong. 2017

[12]Protective Effects of Blueberry Anthocyanins against H2O2-Induced Oxidative Injuries in Human Retinal Pigment Epithelial Cells. Huang, Wu-Yang,Wu, Han,Li, Da-Jing,Song, Jiang-Feng,Xiao, Ya-Dong,Liu, Chun-Quan,Zhou, Jian-Zhong,Huang, Wu-Yang,Sui, Zhong-Quan. 2018

[13]ANTI-FATIGUE EFFECTS OF POLYPHENOLS EXTRACTED FROM ARECA CATECHU L. HUSK AND DETERMINATION OF THE MAIN COMPONENTS BY HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS. Tang, Minmin,Chen, Hua,Zhao, Songlin,Wang, Hui,Qi, Jing. 2016

[14]Antioxidant and Anti-aging Activities of &ITSilybum Marianum&IT Protein Hydrolysate in Mice Treated with D-galactose. Zhu Shu Yun,Yang Jing,Zhou Yue,Zhu Shu Yun,Jiang Ning,Tu Jie. 2017

[15]Functional expression and characterization of Eehinococcus granulosus thioredoxin peroxidase suggests a role in protection against oxidative damage. Li, J,Zhang, WB,Loukas, A,Lin, RY,Ito, A,Zhang, LH,Jones, M,McManus, DP. 2004

[16]Ferulic acid: A natural compound as an efficient feed additive for GIFT (Oreochromis niloticus). Yu, L-J,Wu, F.,Jiang, M.,Yang, C-G,Liu, W.,Tian, J.,Lu, X.,Wen, H.,Yu, L-J. 2018

[17]Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques. Li, Ping,Li, Zhi-Hua,Dzyuba, Boris,Hulak, Martin,Rodina, Marek,Linhart, Otomar,Li, Ping,Li, Zhi-Hua,Dzyuba, Boris. 2010

[18]Isolation of a polysaccharide with antiproliferative, hypoglycemic, antioxidant and HIV-1 reverse transcriptase inhibitory activities from the fruiting bodies of the abalone mushroom Pleurotus abalonus. Tzi Bun Ng,Wang, Chang Rong,Jiang, Yun,Qiao, Wen Tao,Li, Ning,Liu, Fang,Li, Le,Fang, Jin Cen,Wen, Ting Yi. 2011

[19]Hepatoprotective and antioxidant effects of Glycyrrhiza glabra extract against carbon tetrachloride (CCl4)-induced hepatocyte damage in common carp (Cyprinus carpio). Yin, Guojun,Lu, Chengping,Yin, Guojun,Cao, Liping,Xu, Pao,Jeney, Galina,Nakao, Miki. 2011

[20]Hepatoprotective and antioxidant effects of Hibiscus sabdariffa extract against carbon tetrachloride-induced hepatocyte damage in Cyprinus carpio. Yin, Guojun,Cao, Liping,Xu, Pao,Jeney, Galina,Nakao, Miki. 2011

作者其他论文 更多>>

微信小程序