A novel chlorpyrifos hydrolase CPD from Paracoccus sp TRP: Molecular cloning, characterization and catalytic mechanism

文献类型： 外文期刊

第一作者： Fan, Shuanghu

作者： Fan, Shuanghu;Yan, Yanchun;Wang, Junhuan;Wang, Jiayi;Qiao, Cheng;Yang, Ting;Jia, Yang;Zhao, Baisuo;Li, Kang

作者机构：

关键词： Biodegradation;Chlorpyrifos degradation;Chlorpyrifos hydrolysis;Environment;Enzyme purification;Enzyme structure;Esterase;Nucleophilic catalysis;Organophosphate;Persistent pollutants

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.379 ）

ISSN： 0717-3458

年卷期： 2018 年 31 卷

页码：

收录情况： SCI

摘要： Background: Biodegradation is a reliable approach for efficiently eliminating persistent pollutants such as chlorpyrifos. Despite many bacteria or fungi isolated from contaminated environment and capable of degrading chlorpyrifos, limited enzymes responsible for its degradation have been identified, let alone the catalytic mechanism of the enzymes. Results: In present study, the gene cpd encoding a chlorpyrifos hydrolase was cloned by analysis of genomic sequence of Paracoccus sp. TRP. Phylogenetic analysis and BLAST indicated that CPD was a novel member of organophosphate hydrolases. The purified CPD enzyme, with conserved catalytic triad (Ser155-Asp251-His281) and motif Gly-Asp-Ser-Ala-Gly, was significantly inhibited by PMSF, a serine modifier. Molecular docking between CPD and chlorpyrifos showed that Ser155 was adjacent to chlorpyrifos, which indicated that Ser155 may be the active amino acid involved in chlorpyrifos degradation. This speculation was confirmed by site-directed mutagenesis of Ser155Ala accounting for the decreased activity of CPD towards chlorpyrifos. According to the key role of Ser155 in chlorpyrifos degradation and molecular docking conformation, the nucleophilic catalytic mechanism for chlorpyrifos degradation by CPD was proposed. Conclusion: The novel enzyme CPD was capable of hydrolyze chlorpyrifos and Ser155 played key role during degradation of chlorpyrifos. (C) 2017 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.

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