Systematic Analysis of mRNA and miRNA Expression of 3D-Cultured Neural Stem Cells (NSCs) in Spaceflight

文献类型: 外文期刊

第一作者: Cui, Yi

作者: Cui, Yi;Han, Jin;Xiao, Zhifeng;Qi, Yiduo;Zhao, Yannan;Chen, Bing;Liu, Sumei;Wu, Xianming;Dai, Jianwu;Fang, Yongxiang

作者机构:

关键词: spaceflight;RNA-seq;3D culture;NSCs;stemness;differentiation

期刊名称:FRONTIERS IN CELLULAR NEUROSCIENCE ( 影响因子:5.505; 五年影响因子:5.574 )

ISSN: 1662-5102

年卷期: 2018 年 11 卷

页码:

收录情况: SCI

摘要: Recently, with the development of the space program there are growing concerns about the influence of spaceflight on tissue engineering. The purpose of this study was thus to determine the variations of neural stem cells (NSCs) during spaceflight. RNA-Sequencing (RNA-Seq) based transcriptomic profiling of NSCs identified many differentially expressed mRNAs and miRNAs between space and earth groups. Subsequently, those genes with differential expression were subjected to bioinformatic evaluation using gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) and miRNA-mRNA network analyses. The results showed that NSCs maintain greater stemness ability during spaceflight although the growth rate of NSCs was slowed down. Furthermore, the results indicated that NSCs tended to differentiate into neuron in outer space conditions. Detailed genomic analyses of NSCs during spaceflight will help us to elucidate the molecular mechanisms behind their differentiation and proliferation when they are in outer space.

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