Comprehensive Proteomic Analysis of Lysine Acetylation in the Foodborne Pathogen Trichinella spiralis

文献类型: 外文期刊

第一作者: Yang, Yong

作者: Yang, Yong;Bai, Xue;Liu, Xiaolei;Liu, Mingyuan;Yang, Yong;Zhang, Peihao;Cai, Wei;Tong, Mingwei;Cai, Xuepeng;Cai, Xuepeng;Luo, Xuenong;Vallee, Isabelle;Zhou, Yonghua

作者机构:

关键词: lysine acetylation;post-translational modification;lysine acetylation motif;interaction network;Trichinella spiralis

期刊名称:FRONTIERS IN MICROBIOLOGY ( 影响因子:5.64; 五年影响因子:6.32 )

ISSN: 1664-302X

年卷期: 2018 年 8 卷

页码:

收录情况: SCI

摘要: Lysine acetylation is a dynamic and highly conserved post-translational modification that plays a critical role in regulating diverse cellular processes. Trichinella spiralis is a foodborne parasite with a considerable socio-economic impact. However, to date, little is known regarding the role of lysine acetylation in this parasitic nematode. In this study, we utilized a proteomic approach involving anti-acetyl lysine-based enrichment and highly sensitive mass spectrometry to identify the global acetylated proteome and investigate lysine acetylation in T. spiralis. In total, 3872 lysine modification sites were identified in 1592 proteins that are involved in a wide variety of biological processes. Consistent with the results of previous studies, a large number of the acetylated proteins appear to be involved in metabolic and biosynthetic processes. Interestingly, according to the functional enrichment analysis, 29 acetylated proteins were associated with phagocytosis, suggesting an important role of lysine acetylation in this process. Among the identified proteins, 15 putative acetylation motifs were detected. The presence of serine downstream of the lysine acetylation site was commonly observed in the regions surrounding the sites. Moreover, protein interaction network analysis revealed that various interactions are regulated by protein acetylation. These data represent the first report of the acetylome of T. spiralis and provide an important resource for further explorations of the role of lysine acetylation in this foodborne pathogen.

分类号:

  • 相关文献

[1]Proteome-wide lysine acetylation identification in developing rice (Oryza sativa) seeds and protein co-modification by acetylation, succinylation, ubiquitination, and phosphorylation. Meng, Xiaoxi,Lv, Yuanda,Mujahid, Hana,Peng, Zhaohua,Lv, Yuanda,Zhao, Han,Edelmann, Mariola J.,Peng, Xiaojun. 2018

[2]A Genome-Wide Expression Profile of Salt-Responsive Genes in the Apple Rootstock Malus zumi. Liu, Jia,Jiang, Yuzhuang,Xu, Xuefeng,Han, Zhenhai,Kong, Jin,Liu, Jia,Tan, Dunxian,Allan, Andrew C.. 2013

[3]Genome-wide identification, classification, and analysis of heat shock transcription factor family in Chinese cabbage (Brassica rapa pekinensis). Huang, X. Y.,Tao, P.,Li, B. Y.,Wang, W. H.,Yue, Z. C.,Lei, J. L.,Zhong, X. M.,Huang, X. Y.. 2015

[4]Proteomic Quantification of Lysine Acetylation and their Essential Role in Response to Pig Fed Different Protein Levels. Ma, Xianyong. 2018

[5]Global analysis of lysine acetylation in strawberry leaves. Fang, Xianping,Chen, Wenyue,Ruan, Songlin,Ma, Huasheng,Zhao, Yun,Zhang, Hengmu,Yan, Chengqi,Jin, Liang,Cao, Lingling,Zhu, Jun,Cheng, Zhongyi. 2015

[6]A Quantitative Acetylomic Analysis of Early Seed Development in Rice (Oryza sativa L.). Wang, Yifeng,Hou, Yuxuan,Qiu, Jiehua,Li, Zhiyong,Zhao, Juan,Tong, Xiaohong,Zhang, Jian. 2017

[7]A comprehensive quantitative phosphoproteome analysis of rice in response to bacterial blight. Hou, Yuxuan,Qiu, Jiehua,Tong, Xiaohong,Wei, Xiangjin,Huang, Shiwen,Zhang, Jian,Nallamilli, Babi R.,Wu, Weihuai. 2015

[8]The Phosphoproteomic Response of Rice Seedlings to Cadmium Stress. Zhong, Min,Li, Sanfeng,Huang, Fenglin,Qiu, Jiehua,Zhang, Jian,Sheng, Zhonghua,Tang, Shaoqing,Wei, Xiangjin,Hu, Peisong,Zhong, Min,Huang, Fenglin. 2017

[9]Malonylome analysis in developing rice (Oryza saliva) seeds suggesting that protein lysine malonylation is well-conserved and overlaps with acetylation and succinylation substantially. Mujahid, Hana,Meng, Xiaoxi,Xing, Shihai,Peng, Zhaohua,Peng, Xiaojun,Wang, Cailin. 2018

[10]Molecular characterization of a cathepsin F-like protease in Trichinella spiralis. Qu, Zi-gang,Ma, Xue-ting,Li, Wen-hui,Zhang, Nian-zhang,Yue, Long,Cui, Jian-min,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan. 2015

[11]Detection of anti-Trichinella antibodies in serum of experimentally-infected swine by immunochromatographic strip. Fu, B. Q.,Li, W. H.,Gai, W. Y.,Yao, J. X.,Qu, Z. G.,Xie, Z. Z.,Wang, Y. H.,Zhang, D. L.,Blaga, R.. 2013

[12]Cloning and characterization of thioredoxin peroxidases from Trichinella spiralis. Zhang, N. Z.,Liu, J. Y.,Li, W. H.,Li, L.,Qu, Z. G.,Li, T. T.,Cui, J. M.,Yang, Y.,Jia, W. Z.,Fu, B. Q.,Jia, W. Z.,Fu, B. Q..

[13]Proteomic analysis of differentially expressed proteins in the three developmental stages of Trichinella spiralis. Liu, J. Y.,Zhang, N. Z.,Li, W. H.,Li, L.,Yan, H. B.,Qu, Z. G.,Li, T. T.,Cui, J. M.,Yang, Y.,Jia, W. Z.,Fu, B. Q.,Jia, W. Z.,Fu, B. Q..

[14]Use of mitochondrial RNA genes for the differentiation of four Trichinella species by multiplex PCR amplification. Blaga, R.,Fu, BaoQuan,Le Rhun, D.,Le Naour, E.,Heckman, A.,Zocevic, A.,Boireau, P.,Liu, MingYuan.

[15]High-level expression and characterization of two serine protease inhibitors from Trichinella spiralis. Zhang, Zhaoxia,Mao, Yixian,Li, Da,Zhang, Yvhan,Li, Wei,Li, Li,Lu, Yixin,Jia, Honglin,Zheng, Jun.

[16]Cloning, identification, and bioinformatics analysis of a putative aquaporin TsAQP from Trichinella spiralis. Cui, J. M.,Zhang, N. Z.,Li, W. H.,Yan, H. B.,Fu, B. Q.,Fu, B. Q.. 2015

[17]Identification of stage-specifically expressed genes of Trichinella spiralis by suppression subtractive hybridization. Liu, M. Y.,Wang, X. L.,Fu, B. Q.,Li, C. Y.,Wu, X. P.,Le Rhun, D.,Chen, Q. J.,Boireau, P.. 2007

[18]Study of the 49 kDa excretory-secretory protein gene of Trichinella nativa and Trichinella spiralis. Zheng, B. L.,Xiao, L. H.,Wang, X. R.,Li, D. M.,Lu, Y. X.,Zhang, Y.,Yan, Q. B.,Song, M. X.. 2007

作者其他论文 更多>>