Chitosan oligosaccharides with degree of polymerization 2-6 induces apoptosis in human colon carcinoma HCT116 cells

文献类型： 外文期刊

第一作者： Zou, Pan

作者： Zou, Pan;Yang, Xin;Zhai, Xingchen;Wang, Jing;Zou, Pan;Yuan, Shoujun;Yang, Xin;Zhai, Xingchen;Wang, Jing;Zou, Pan;Yuan, Shoujun

作者机构：

关键词： Chitosan oligosaccharides;Degree of polymerization;Colon cancer;HCT116;Apoptosis

期刊名称：CHEMICO-BIOLOGICAL INTERACTIONS （ 影响因子：5.192； 五年影响因子：4.61 ）

ISSN： 0009-2797

年卷期： 2018 年 279 卷

页码：

收录情况： SCI

摘要： Colon cancer is the third most common cancer, and yet there is a lack of effective therapeutic method with low side effects. Chitosan oligosaccharides (COS) is derived from chitosan after chitin deacetylation, and attracts more interests due to smaller molecular weight and soluble property. Previously, COS, mainly absorbed through intestinal epithelia, has been reported to exhibit many bioactivities, especially its anti-tumor effect. Recent references pay little attention to molecular weight distribution which is crucial for understanding its biological behavior. Here, we studied reducing sugar content and degree of polymerization (DP) of COS. 86.73% reducing sugar exists in COS sample and the content of chitosan fractions with 2-6 is 85.8%. COS suppressed the growth of HCT116 cells in vitro and in vivo, and the inhibition rate of tumor weight in vivo was high up to 58.6%. Moreover, the morphology observation, flow cytometry analysis and mRNA expression were applied to study the apoptosis related mechanism. COS treatment promoted mitosis, late stage apoptosis and S cell cycle arrest in HCT116 cells, and enhanced the mRNA expression of BAK and reduce BCL-2 and BCL-x(L). These findings may provide an important clue for clinical applications of COS as anti-tumor drug or pharmaceutic adjuvant in the future.

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