Constitutive expression of feedback-insensitive cystathionine gamma-synthase increases methionine levels in soybean leaves and seeds

文献类型： 外文期刊

第一作者： Yu Yang

作者： Yu Yang;Hou Wen-sheng;Sun Shi;Wu Cun-xiang;Song Shi-kui;Han Tian-fu;Hacham, Yael;Matityahu, Ifat;Amir, Rachel;Hacham, Yael;Amir, Rachel

作者机构：

关键词： soybean (Glycine max (L.) Merr.);constitutive expression;cystathionine gamma-synthase (CGS);methionine content;storage protein

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2018 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Soybean (Glycine max (L.) Merr.) is a major crop that provides plant-origin protein and oil for humans and livestock. Although the soybean vegetative tissues and seeds provide a major source of high-quality protein, they suffer from low concentration of an essential sulfur-containing amino acid, methionine, which significantly limits their nutritional quality. The level of methionine is mainly controlled by the first unique enzyme of methionine synthesis, cystathione gamma-synthase (CGS). Aiming to elevate methionine level in vegetative tissues and seeds, we constitutively over-expressed a feedback-insensitive Arabidopsis CGS (AtD-CGS) in soybean cultivars, Zigongdongdou (ZD) and Jilinxiaoli 1 (JX). The levels of soluble methionine increased remarkably in leaves of transgenic soybeans compared to wild-type plants (6.6-and 7.3-fold in two transgenic ZD lines, and 3.7-fold in one transgenic JX line). Furthermore, the total methionine contents were significantly increased in seeds of the transgenic ZD lines (1.5-to 4.8-fold increase) and the transgenic JX lines (1.3-to 2.3-fold increase) than in the wild type. The protein contents of the transgenic soybean seeds were significantly elevated compared to the wild type, suggesting that the scarcity of methionine in soybeans may limit protein accumulation in soybean seeds. The increased protein content did not alter the profile of major storage proteins in the seeds. Generally, this study provides a promising strategy to increase the levels of methionine and protein in soybean through the breeding programs.

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