Construction of Salmonella Pullorum ghost by co-expression of lysis gene E and the antimicrobial peptide SMAP29 and evaluation of its immune efficacy in specific-pathogen-free chicks

文献类型: 外文期刊

第一作者: Tian Qiu-feng

作者: Tian Qiu-feng;Zhou Wei;Si Wei;Yi Fei;Hua Xin;Chen Li-ping;Liu Si-guo;Yu Shen-ye;Tian Qiu-feng;Zhou Wei;Yi Fei;Yue Min

作者机构:

关键词: Salmonella Pullorum;bacterial ghost;antimicrobial peptide;immune response;immune protection

期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )

ISSN: 2095-3119

年卷期: 2018 年 17 卷 1 期

页码:

收录情况: SCI

摘要: In this study, a safety enhanced Salmonella Pullorum (S. Pullorum) ghost was constructed using an antimicrobial peptide gene, and evaluated for its potential as a Pullorum disease (PD) vaccine candidate. The antimicrobial peptide SMAP29 was co-expressed with lysis gene E to generate S. Pullorum ghosts. No viable bacteria were detectable either in the fermentation culture after induction of gene E-and SMAP29-mediated lysis for 24 h or in the lyophilized ghost products. Specific-pathogenfree (SPF) chicks were intraperitoneally immunized with ghosts at day 7 of age and no mortality, clinical symptoms or signs of PD such as anorexia, depression and diarrhea were observed. On challenge with a virulent S. Pullorum strain at 4 wk post-immunization, a comparatively higher level of protection was observed in the S. Pullorum ghost immunized chickens with a minimum of pathological lesions and bacterial loads compared to the birds in inactivated vaccine groups. In addition, immunization with the S. Pullorum ghosts induced a potent systemic IgG response and was associated with significantly increased levels of cytokine IFN-gamma and IL-4 and relative percentages of CD4(+) and CD8(+) T lymphocytes. Our results indicate that SMAP29 can be employed as a new secondary lethal protein to enhance the safety of bacterial ghosts, and to prepare a non-living bacterial vaccine candidate that can prevent PD in chickens.

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