Transcriptomic analysis of mouse liver reveals a potential hepato-enteric pathogenic mechanism in acute Toxoplasma gondii infection

文献类型： 外文期刊

第一作者： He, Jun-Jun

作者： He, Jun-Jun;Ma, Jun;Song, Hui-Qun;Huang, Si-Yang;Zhu, Xing-Quan;Elsheikha, Hany M.;Ma, Jun;Huang, Si-Yang;Zhu, Xing-Quan

作者机构：

关键词： Toxoplasma gondii;Mouse liver;Transcriptome;RNA-seq;Liver function

期刊名称：PARASITES & VECTORS （ 影响因子：3.876； 五年影响因子：3.959 ）

ISSN： 1756-3305

年卷期： 2016 年 9 卷

页码：

收录情况： SCI

摘要： Background: Toxoplasma gondii is a worldwide spread pathogen which can infect all tissues of its host. The transcriptomic responses of infected brain and spleen have been reported. However, our knowledge of the global transcriptomic change in infected liver is limited. Additionally, T. gondii infection represents a highly dynamic process involving complex biological responses of the host at many levels. Herein, we describe such processes at a global level by discovering gene expression changes in mouse livers after acute infection with T. gondii ToxoDB#9 strain. Results: Global transcriptomic analysis identified 2,758 differentially expressed transcripts in infected liver, of which 1,356 were significantly downregulated and 1,402 upregulated. GO and KEGG database analyses showed that host immune responses were upregulated, while the metabolic-related processes/pathways were downregulated, especially xenobiotic metabolism, fatty acid metabolism, energy metabolism, and bile biosynthesis and secretion. The metabolism of more than 800 chemical compounds including anti-Toxoplasma prescribed medicines were predicted to be modulated during acute T. gondii infection due to the downregulation of enzymes involved in xenobiotic metabolism. Conclusions: To the best of our knowledge, this is the first global transcriptomic analysis of mouse liver infected by T. gondii. The present data indicate that during the early stage of liver infection, T. gondii can induce changes in liver xenobiotic metabolism, upregulating inflammatory response and downregulating hepatocellular PPAR signaling pathway, altering host bile biosynthesis and secretion pathway; these changes could enhance host intestinal dysbacteriosis and thus contribute to the pathological changes of both liver and intestine of infected mice. These findings describe the biological changes in infected liver, providing a potential mechanistic pathway that links hepatic and intestinal pathologies to T. gondii infection.

分类号：

  • 相关文献

[1]Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection. Cong, Wei,Zhang, Xiao-Xuan,He, Jun-Jun,Li, Fa-Cai,Zhu, Xing-Quan,Cong, Wei,Elsheikha, Hany M.. 2017

[2]Dual Identification and Analysis of Differentially Expressed Transcripts of Porcine PK-15 Cells and Toxoplasma gondii during in vitro Infection. Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Zhou, Dong-Hui,Liu, Qing,Zhu, Xing-Quan,Elsheikha, Hany M.,Zhu, Xing-Quan. 2016

[3]De novo assembly of pen shell (Atrina pectinata) transcriptome and screening of its genic microsatellites. Sun, Xiujun,Li, Dongming,Liu, Zhihong,Zhou, Liqing,Wu, Biao,Yang, Aiguo,Sun, Xiujun,Li, Dongming,Liu, Zhihong,Zhou, Liqing,Wu, Biao,Yang, Aiguo. 2017

[4]De novo Transcriptome Assembly of Chinese Kale and Global Expression Analysis of Genes Involved in Glucosinolate Metabolism in Multiple Tissue. Wu, Shuanghua,Lei, Jianjun,Chen, Guoju,Cao, Bihao,Chen, Changming,Chen, Hancai. 2017

[5]De Novo Transcriptome Sequencing and the Hypothetical Cold Response Mode of Saussurea involucrata in Extreme Cold Environments. Li, Jin,Liu, Hailiang,Xia, Wenwen,Mu, Jianqiang,Feng, Yujie,Liu, Ruina,Wang, Aiying,Lin, Zhongping,Zhu, Jianbo,Chen, Xianfeng,Liu, Hailiang,Yan, Panyao,Chen, Xianfeng,Lin, Zhongping,Guo, Yong. 2017

[6]Transcriptome sequencing and analysis of major genes involved in calcium signaling pathways in pear plants (Pyrus calleryana Decne.). Lin, Jing. 2015

[7]De Novo Assembly and Characterization of Pericarp Transcriptome and Identification of Candidate Genes Mediating Fruit Cracking in Litchi chinensis Sonn.. Li, Wei-Cai,Zhang, Hong-Na,Shi, Sheng-You,Liu, Li-Qin,Shu, Bo,Liang, Qing-Zhi,Xie, Jiang-Hui,Wei, Yong-Zan,Wu, Jian-Yang. 2014

[8]Comprehensive analysis of RNA-seq data reveals the complexity of the transcriptome in Brassica rapa. Tong, Chaobo,Yu, Jingyin,Huang, Junyan,Dong, Caihua,Hua, Wei,Liu, Shengyi,Wang, Xiaowu,Wu, Jian,Li, Wanshun. 2013

[9]Transcriptome analysis of Cucumis sativus infected by Cucurbit chlorotic yellows virus. Sun, Xinyan,Wang, Zhenyue,Li, Honglian,Han, Weili,Shi, Yan,Gu, Qinsheng. 2017

[10]DE NOVO TRANSCRIPTOME ANALYSIS OF MULBERRY (MORUS L.) UNDER DROUGHT STRESS USING RNA-SEQ TECHNOLOGY. Wang, Heng,Tong, Wei,Feng, Li,Jiao, Qian,Long, Li,Fang, Rongjun,Zhao, Weiguo,Long, Li,Zhao, Weiguo,Fang, Rongjun,Zhao, Weiguo.

[11]Comparative transcriptome analysis of latex from rubber tree clone CATAS8-79 and PR107 reveals new cues for the regulation of latex regeneration and duration of latex flow. Chao, Jinquan,Chen, Yueyi,Wu, Shaohua,Tian, Wei-Min. 2015

[12]RNA-Seq of the xylose-fermenting yeast Scheffersomyces stipitis cultivated in glucose or xylose. Yuan, Tiezheng,Meng, Kun,Yang, Peilong,Shi, Pengjun,Yao, Bin,Ren, Yan,Feng, Yun,Wang, Shaojing,Wang, Lei,Ren, Yan,Feng, Yun,Wang, Shaojing,Wang, Lei,Xie, Daoxin.

[13]Transcriptome analysis of Brassica napus pod using RNA-Seq and identification of lipid-related candidate genes. Xu, Hai-Ming,Kong, Xiang-Dong,Chen, Fei,Huang, Ji-Xiang,Zhao, Jian-Yi,Lou, Xiang-Yang. 2015

[14]Comparative Transcriptome Analysis Reveals Heat-Responsive Genes in Chinese Cabbage (Brassica rapa ssp chinensis). Wang, Aihua,Huang, Xingxue,Li, Xia,Zhou, Guolin,Hu, Jihong,Hu, Jihong,Yan, Zhixiang. 2016

[15]Analysis of global gene expression profiles to identify differentially expressed genes critical for embryo development in Brassica rapa. Zhang, Yu,Peng, Lifang,Wu, Ya,Shen, Yanyue,Wang, Jianbo,Wu, Xiaoming.

[16]RNA-seq analysis of bovine intramuscular, subcutaneous and perirenal adipose tissues. Sheng, Xihui,Ni, Hemin,Liu, Yunhai,Guo, Yong,Li, Junya,Zhang, Lupei.

[17]Transcriptome analysis of rice root heterosis by RNA-Seq. Feng, Yue,Zhan, Xiaodeng,Shen, Xihong,Wu, Weiming,Zhang, Yingxin,Chen, Daibo,Cao, Liyong,Cheng, Shihua,Wang, Huimin,Dai, Gaoxing,Yang, Zhanlie. 2013

[18]Transcriptome sequencing analysis of porcine granulosa cells treated with an anti-inhibin antibody. Lei, Mingming,Cai, Liuping,Li, Hui,Chen, Zhen,Shi, Zhendan. 2017

[19]Transcriptomic analysis of global changes in cytokine expression in mouse spleens following acute Toxoplasma gondii infection. He, Jun-Jun,Ma, Jun,Song, Hui-Qun,Zhou, Dong-Hui,Wang, Jin-Lei,Huang, Si-Yang,Zhu, Xing-Quan,Ma, Jun,Zhu, Xing-Quan.

[20]Comparative Transcriptome Analysis Reveals Differential Transcription in Heat-susceptible and Heat-tolerant Pepper (Capsicum annum L.) Cultivars under Heat Stress. Li, Tao,Xu, Xiaowan,Li, Ying,Wang, Hengming,Li, Zhiliang,Li, Zhenxing,Li, Tao,Xu, Xiaowan.

作者其他论文 更多>>

微信小程序