Identification of ABCC2 as a binding protein of Cry1Ac onbrush border membrane vesicles from Helicoverpa armigera by an improved pull-down assay

文献类型： 外文期刊

第一作者： Wang, Zeyu

作者： Wang, Zeyu;Zhou, Xueping;Zhou, Zishan;Liu, Yuxiao;Liang, Gemei;Shu, Changlong;Song, Fuping;Zhou, Xueping;Zhang, Jie;Bravo, Alejandra;Soberon, Mario

作者机构：

关键词： ABCC2;alkaline phosphatase;aminopeptidase-N;Bacillus thuringiensis;cadherin;Cry1Ac;Helicoverpa armigera

期刊名称：MICROBIOLOGYOPEN （ 影响因子：3.139； 五年影响因子：3.503 ）

ISSN： 2045-8827

年卷期： 2016 年 5 卷 4 期

页码：

收录情况： SCI

摘要： Cry1Ac toxin-binding proteins from Helicoverpa armigera brush border membrane vesicles were identified by an improved pull-down method that involves coupling Cry1Ac to CNBr agarose combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). According to the LC-MS/MS results, Cry1Ac toxin could bind to six classes of aminopeptidase-N, alkaline phosphatase, cadherin-like protein, ATP-binding cassette transporter subfamily C protein (ABCC2), actin, ATPase, polycalin, and some other proteins not previously characterized as Cry toxin-binding molecules such as dipeptidyl peptidase or carboxyl/choline esterase and some serine proteases. This is the first report that suggests the direct binding of Cry1Ac toxin to ABCC2 in H.armigera.

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