文献类型： 外文期刊

第一作者： Tian Lei

作者： Tian Lei;Su Xiao-Yun;Zhang Zhen-Feng;Huang Xi-Tai

作者机构：

关键词： DNA IV;restriction enzyme;DNA topoisomerase;DNA nodes;atomic force microscopy (AFM)

期刊名称：PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS （ 影响因子：0.351； 五年影响因子：0.272 ）

ISSN： 1000-3282

年卷期： 2016 年 43 卷 8 期

页码：

收录情况： SCI

摘要： Combinatorial analyses including enzymatic analysis and atomic force microscopy (AFM) were used to gain more insights into structure of the alkali-denatured plasmid pBR322 DNA (DNA IV). Among the restriction enzymes tested, Pst I was able to cleave the pBR322 DNA IV molecules, indicative of the existence of an intact restriction site in the denatured DNA. AFM images revealed that Pst I-treated DNA N molecules contained an individual node. The contour length of the condensed molecules was decreased by about 11% compared to DNA N. Intriguingly, the Escherichia coli topoisomerase N, a type II topoisomerase, also introduced nodes into DNA N while keeping the molecules in a closed form. The two types of DNA were similar to each other in terms of the contour length and the size of the node based on the AFM analysis. These findings suggest that specific base-paired regions exist in DNA N, and that cleavages at these regions may lead to DNA node.

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