A single mutation in the P-BC loop of VP2 is involved in the in vitro replication of infectious bursal disease virus

文献类型： 外文期刊

第一作者： Qi, Xiaole

作者： Qi, Xiaole;Gao, Xiang;Lu, Zhen;Zhang, Lizhou;Wang, Yongqiang;Gao, Li;Gao, Yulong;Li, Kai;Gao, Honglei;Liu, Changjun;Cui, Hongyu;Zhang, Yanping;Wang, Xiaomei;Wang, Xiaomei

作者机构：

关键词： infectious bursal disease virus (IBDV);VP2;P-BC;replication

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：6.038； 五年影响因子：4.754 ）

ISSN： 1674-7305

年卷期： 2016 年 59 卷 7 期

页码：

收录情况： SCI

摘要： To test whether amino acid mutations in the P-BC and P-HI loops of VP2 are involved in the replication and virulence of infectious bursal disease virus (IBDV), a pair of viruses, namely the moderately virulent IBDV (rGx-F9VP2) and the attenuated strain (rGt), were used. Residue mutations A222P (P-BC) and S330R (P-HI), selected by sequence comparison, were introduced individually into rGx-F9VP2 by using a reverse genetics system. In addition, the reverse mutation of either P222A or R330S was introduced into rGt. The four modified viruses were then rescued and evaluated in vitro (CEF cells) and in vivo (SPF chickens). Results showed that A222P elevated the replication efficiency of rGx-F9VP2 while P222A reduced that of rGt in CEF cells. A mutation at residue 330 did not alter IBDV replication. In addition, animal experiments showed that a single mutation at either residue 222 or 330 did not significantly influence the virulence of IBDV. In conclusion, residue 222 in PBC of VP2 is involved in the replication efficiency of IBDV in vitro but does not affect its virulence in vivo, further facilitating our understanding of the gene-function of IBDV.

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