Detection of the Diversity of Cytoplasmic Male Sterility Sources in Broccoli (Brassica Oleracea var. Italica) Using Mitochondrial Markers

文献类型： 外文期刊

第一作者： Shu, Jinshuai

作者： Shu, Jinshuai;Liu, Yumei;Li, Zhansheng;Zhang, Lili;Fang, Zhiyuan;Yang, Limei;Zhuang, Mu;Zhang, Yangyong;Lv, Honghao

作者机构：

关键词： broccoli;cytoplasmic male sterility;origin;mitochondrial markers;diversity

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： Broccoli (Brassica oleracea var. italica) is an important commercial vegetable crop. As part of an efficient pollination system, cytoplasmic male sterility (CMS) has been widely used for broccoli hybrid production. Identifying the original sources of CMS in broccoli accessions has become an important part of broccoli breeding. In this study, the diversity of the CMS sources of 39 broccoli accessions, including 19 CMS lines and 20 hybrids, were analyzed using mitochondria' markers. All CMS accessions contained the ogu orf/38-related DNA fragment and the key genes of nap CMS, pol CMS, and tour CMS were not detected. The 39 CMS accessions were divided into five groups using six orf/38-related and two simple sequence repeat markers. We observed that ogu CMS R3 constituted 79.49% of the CMS sources. CMS6 and CMS26 were differentiated from the other accessions using a specific primer. CMS32 was distinguished from the other accessions based on a 78-nucleotide deletion at the same locus as the orf/38-related sequence. When the coefficient was about 0.90, five CMS accessions (13CMS6, 13CMS23, 13CMS24, 13CMS37, and 13CMS39) exhibiting abnormal floral organs with poor seed setting were grouped together. The polymerase chain reaction amplification profiles for these five accessions differed from those of the other accessions. We identified eight useful molecular markers that can be used to detect CMS types during broccoli breeding. Our data also provide important information relevant to future studies on the possible origins and molecular mechanisms of CMS in broccoli.

分类号：

  • 相关文献

[1]Isolation, characterization and phylogenetic analysis of the resistance gene analogues (RGAs) in banana (Musa spp.). Pei, Xinwu,Li, Shengjun,Jiang, Ying,Zhang, Yongqiang,Wang, Zhixing,Jia, Shirong.

[2]Effect of Different Pre-Cooling, Packaging and Cold Storage Treatments on Quality of Broccoli. Yan, L.,Liu, S.. 2012

[3]EFFECT OF DIFFERENT PRE-COOLING AND TRANSPORTATION METHODS ON QUALITY OF BROCCOLI. Yan, L.,Liu, S.,Wang, Z.,Rao, X.. 2011

[4]Effects of Red Light-Emitting Diode (LED) on the Postharvest Yellowing Change of Broccoli. Zhang Na,Yan Rui-xiang,Guan Wen-qiang,Wang Chao. 2016

[5]Determination of sulforaphane in broccoli and cabbage by high-performance liquid chromatography. Liang, H,Yuan, QP,Dong, HR,Liu, YM. 2006

[6]Potential of a strain of the entomopathogenic fungus Beauveria bassiana (Hypocreales: Cordycipitaceae) as a biological control agent against western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae). Gao, Yulin,Wang, Jing,Xu, Xuenong,Lei, Zhongren,Reitz, Stuart R.. 2012

[7]Comparative Transcriptome Analysis between Broccoli (Brassica oleracea var. italica) and Wild Cabbage(Brassica macrocarpa Guss.) in Response to Plasmodiophorabrassicae during Different Infection Stages. Zhang, Xiaoli,Liu, Yumei,Fang, Zhiyuan,Li, Zhansheng,Yang, Limei,Zhuang, Mu,Zhang, Yangyong,Lv, Honghao. 2016

[8]Laboratory and greenhouse evaluation of a new entomopathogenic strain of Beauveria bassiana for control of the onion thrips Thrips tabaci. Gao, Yulin,Xu, Xuenong,Zhang, Yaping,Wang, Jing,Lei, Zhongren,Smagghe, Guy.

[9]A generic SSR marker closely linked to a dominant genic male sterility gene (DGMs79-399-3) in broccoli (Brassica oleracea var. italica). Shu, Jinshuai,Liu, Yumei,Li, Zhansheng,Zhang, Lili,Fang, Zhiyuan,Yang, Limei,Zhuang, Mu,Zhang, Yangyong,Lv, Honghao.

[10]Genetic analysis of floral organ size in broccoli x cabbage via a mixed inheritance model of a major gene plus polygene. Shu, J. S.,Liu, Y. M.,Li, Z. S.,Zhang, L. L.,Fang, Z. Y.,Yang, L. M.,Zhuang, M.,Zhang, Y. Y.,Lv, H. H.. 2016

[11]Effect of Se treatment on the volatile compounds in broccoli. Lv, Jiayu,Wu, Jie,Zuo, Jinhua,Fan, Linlin,Shi, Junyan,Gao, Lipu,Wang, Qing,Wu, Jie,Li, Miao,Wu, Jie,Li, Miao. 2017

[12]Effect of controlled atmospheres with varying O-2/CO2 levels on the postharvest senescence and quality of broccoli (Brassica oleracea L. var. italica) florets. Guo, Yanyin,Li, Ling,Wang, Yuanyuan,Zhao, Hongqing,Gao, Zhaoyin,Hu, Meijiao,Li, Min,Zhang, Zhengke. 2013

[13]VARIATION OF SULFORAPHANE LEVELS IN BROCCOLI (BRASSICA OLERACEA VAR. ITALICA) DURING FLOWER DEVELOPMENT AND THE ROLE OF GENE AOP2. Li, Zhansheng,Liu, Yumei,Fang, Zhiyuan,Yang, Limei,Zhuang, Mu,Zhang, Yangyong,Sun, Peitian,Zhao, Wen.

[14]Temperature abuse timing affects the rate of quality deterioration of postharvest broccoli during different pre-storage stages. Zhu, Yongqing,Luo, Fangyao,Gao, Jia,Zhu, Yongqing,Si, Yuezhou,Yan, Shoulei. 2018

[15]Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance. Jiang Ming,He Cai-ming,Miao Li-xiang,Zhang Yu-chao. 2012

[16]An efficient shoot regeneration system and Agrobacterium-mediated transformation with codA gene in a doubled haploid line of broccoli. Sheng, Xiaoguang,Gu, Honghui,Yu, Huifang,Wang, Jiansheng,Zhao, Zhenqing,Qi, Zirong. 2016

[17]HEAVY METAL CONTENT IN BR CCOLI (BRASSICA OLERACEA L. VAR. ITALICA PLENCK) AND POTENTIAL HEALTH RISK IN ZHEJIANG PROVINCE, CHINA. Sun, Caixia,Zhang, Qi,Xiao, Wendan,Xu, Ping,Xiang, Shuo. 2017

[18]Exogenous sodium nitroprusside treatment of broccoli florets extends shelf life, enhances antioxidant enzyme activity, and inhibits chlorophyll-degradation. Shi, Junyan,Gao, Lipu,Zuo, Jinhua,Wang, Qing,Wang, Qian,Fan, Linlin,Shi, Junyan,Gao, Lipu,Zuo, Jinhua,Wang, Qing,Wang, Qian,Fan, Linlin.

[19]Changes in Sulforaphane and Selenocysteine Methyltransferase Transcript Levels in Broccoli Treated with Sodium Selenite. Huang, K.,Wu, Q. Y.,Liu, M. Y.,Huang, K.,Wu, Q. Y.,Liu, M. Y.,Zhang, S.,Xiao, W. J.,Lin, J. C.,Yan, J. Y..

[20]Genotypic variation of glucosinolates in broccoli (Brassica oleracea var. italica) florets from China. Wang, Jiansheng,Gu, Honghui,Yu, Huifang,Zhao, Zhenqing,Sheng, Xiaoguang,Zhang, Xiaohui.

作者其他论文 更多>>

微信小程序