Differential gene expression in porcine SK6 cells infected with wild-type and SAP domain-mutant foot-and-mouth disease virus

文献类型： 外文期刊

第一作者： Ni, Zixin

作者： Ni, Zixin;Yang, Fan;Cao, Weijun;Zhang, Xiangle;Jin, Ye;Mao, Ruoqing;Du, Xiaoli;Li, Weiwei;Guo, Jianhong;Liu, Xiangtao;Zhu, Zixiang;Zheng, Haixue;Ni, Zixin

作者机构：

关键词： Foot-and-mouth disease virus (FMDV);leader protein;SAP region;transcriptome analysis

期刊名称：VIROLOGICA SINICA （ 影响因子：4.327； 五年影响因子：4.08 ）

ISSN： 1674-0769

年卷期： 2016 年 31 卷 3 期

页码：

收录情况： SCI

摘要： Foot-and-mouth disease virus (FMDV) is the causative agent of a highly contagious disease in livestock. The viral proteinase L-pro of FMDV is involved in pathogenicity, and mutation of the L-pro SAP domain reduces FMDV pathogenicity in pigs. To determine the gene expression profiles associated with decreased pathogenicity in porcine cells, we performed transcriptome analysis using next-generation sequencing technology and compared differentially expressed genes in SK6 cells infected with FMDV containing L-pro with either a wild-type or mutated version of the SAP domain. This analysis yielded 1,853 genes that exhibited a >= 2-fold change in expression and was validated by real-time quantitative PCR detection of several differentially expressed genes. Many of the differentially expressed genes correlated with antiviral responses corresponded to genes associated with transcription factors, immune regulation, cytokine production, inflammatory response, and apoptosis. Alterations in gene expression profiles may be responsible for the variations in pathogenicity observed between the two FMDV variants. Our results provided genes of interest for the further study of antiviral pathways and pathogenic mechanisms related to FMDV L-pro.

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