A protocol of homozygous haploid callus induction from endosperm of Taxus chinensis Rehd. var. mairei

文献类型: 外文期刊

第一作者: Li, Yan-Lin

作者: Li, Yan-Lin;Huang, San-Wen;Xiong, Xing-Yao;Li, Yan-Lin;Huang, San-Wen;Bu, Feng-Jiao;Lin, Tao;Zhang, Zhong-Hua;Xiong, Xing-Yao;Zhang, Jia-Yin

作者机构:

关键词: Taxus;Haploid;Endosperm;Callus initiation;Homozygote;SNP mark

期刊名称:SPRINGERPLUS ( 影响因子:1.13; 五年影响因子:1.29 )

ISSN: 2193-1801

年卷期: 2016 年 5 卷

页码:

收录情况: SCI

摘要: Obtainment and characterization of the novel endosperm callus of Taxus chinensis Rehd. var. mairei are valuable for haploid breeding, genome, and functional genome in Taxus. Callus was obtained by hydropriming with sterile water for 3 days and suitable medium composition. The highest callus induction (70.89 %) and lower browning ratio (7.95 %) were obtained from Gamborg (B-5) medium supplemented with 30 g l(-1) of sucrose, 2.5 mg l(-1) of 2,4-dichlorophenoxyacetic (2,4-D), 0.5 mg l(-1) of 6-benzylademine (6-BA) and 7 g l(-1) of agar under dark conditions. The auxin of 2,4-D had a better efficiency of callus induction than naphthylacetic acid, and over 1 mg l(-1) of 6-BA was inhibitory to the callogensis of endosperm. The endosperm callus was haploid which was detectable by the flow cytometry. The genome block of homozygosity of callus was homozygous which was indicated by PCR-based SNP marks. The homozygous haploid of endosperm callus in vitro culture may be useful tools for taxoid-metabolism of gene engineering and bio-fermentation engineering.

分类号:

  • 相关文献

[1]Androgenesis, gynogenesis, and parthenogenesis haploids in cucurbit species. Dong, Yan-Qi,Liu, Xi-Cun,Huang, Jin-Hua,Wang, Wen-Ying,Tang, Zhen-Hai,Zhao, Wei-Xing,Li, Xiao-Hui,Gao, Ning-Ning,Xu, Xiao-Li.

[2]Haploid Induction via In vitro Gynogenesis in Tomato (Solanum lycopersicum L.). Zhao He,Wang Xiao-xuan,Du Yong-chen,Zhu De-wei,Guo Yan-mei,Gao Jian-chang,Li Fei,Snyder, John C.. 2014

[3]Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.). Li, Jin-Rong,Zhuang, Fei-Yun,Ou, Cheng-Gang,Hu, Hong,Zhao, Zhi-Wei,Mao, Ji-Hua.

[4]Comparative proteomic analyses of two Taxus species (Taxus X media and Taxus mairei) reveals variations in the metabolisms associated with paclitaxel and other metabolites. Juan Hao,Shen, Chenjia,Hong Guo,Xinai Shi,Ye Wang,Qinghua Wan,Yao-Bin Song,Lei Zhang,Ming Dong,Chenjia Shen. 2017

[5]Sequence-related amplified polymorphism (SRAP) marker as a new method for identification of endophytic fungi from Taxus. Ren, Na,Liu, Jiajia,Yang, Dongliang,Hong, Juan,Chen, Jianhua,Luan, Mingbao. 2012

[6]Identification of potential genes that contributed to the variation in the taxoid contents between two Taxus species (Taxus media and Taxus mairei). Yu, Chunna,Guo, Hong,Zhang, Yangyang,Song, Yaobin,Pi, Erxu,Dong, Ming,Wang, Huizhong,Shen, Chenjia,Yu, Chunna,Guo, Hong,Wang, Huizhong,Shen, Chenjia,Song, Yaobin,Dong, Ming,Yu, Chenliang,Zhang, Lei,Zheng, Bingsong. 2017

[7]Genome-wide high-resolution mapping of DNA methylation identifies epigenetic variation across embryo and endosperm in Maize (Zea may). Wang, Pengfei,Ma, Chuanxi,Wang, Pengfei,Xia, Han,Zhang, Ye,Zhao, Shuzhen,Zhao, Chuanzhi,Hou, Lei,Li, Changsheng,Li, Aiqin,Wang, Xingjun. 2015

[8]Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds. Xu, Heng-Heng,Liu, Shu-Jun,Wang, Wei-Qing,Song, Song-Quan,Li, Ni,Moller, Ian M.. 2016

[9]OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm. Wang, Jie-Chen,Cai, Xiu-Ling,Xu, Heng,Zhu, Ying,Liu, Qiao-Quan. 2013

[10]Du1, encoding a novel Prp1 protein, regulates starch biosynthesis through affecting the splicing of Wx(b) supercript stop pre-mRNAs in rice (Oryza sativa L.). Zeng, Dali,Yan, Meixian,Wang, Yonghong,Liu, Xinfang,Qian, Qian,Li, Jiayang.

[11]High-Throughput Sequencing of Small RNA Transcriptomes in Maize Kernel Identifies miRNAs Involved in Embryo and Endosperm Development. Xing, Lijuan,Zhu, Ming,Zhang, Min,Li, Wenzong,Zou, Junjie,Wang, Lei,Xu, Miaoyun,Zhu, Ming,Jiang, Haiyang. 2017

[12]Identification and characterization of the zinc-regulated transporters, iron-regulated transporter-like protein (ZIP) gene family in maize. Li, Suzhen,Huang, Yaqun,Zhu, Liying,Zhao, Yongfeng,Guo, Jinjie,Chen, Jingtang,Li, Suzhen,Zhou, Xiaojin,Zhang, Shaojun,Chen, Rumei. 2013

[13]Control of Rice Embryo Development, Shoot Apical Meristem Maintenance, and Grain Yield by a Novel Cytochrome P450. Yang, Weibing,Gao, Mingjun,Yin, Xin,Zeng, Longjun,Li, Qun,He, Zuhua,Yang, Weibing,Gao, Mingjun,Yin, Xin,Zeng, Longjun,Li, Qun,He, Zuhua,Liu, Jiyun,Wang, Junmin,Zhang, Xiaoming,Xu, Yonghan,Zhang, Shubiao.

[14]Gene duplication confers enhanced expression of 27-kDa gamma-zein for endosperm modification in quality protein maize. Liu, Hongjun,Sun, Chuanlong,Zheng, Xixi,Yuan, Ningning,Li, Changsheng,Zhang, Zhiyong,Deng, Yiting,Wang, Jiechen,Wu, Yongrui,Shi, Junpeng,Lai, Jinsheng,Shi, Junpeng,Lai, Jinsheng,Gong, Hao,Huang, Xuehui,Feng, Qi,Han, Bin,Fan, Xingming,Qiu, Fazhan,Pan, Guangtang,Pan, Guangtang.

[15]Gene expression in a starch synthase IIa mutant of barley: changes in the level of gene transcription and grain composition. Clarke, B.,Morell, M. K.,Jenkins, C. L. D.,Li, Z.,Liang, R.,Bird, A. R..

[16]Parent-of-Origin-Effect rough endosperm Mutants in Maize. Bai, Fang,Daliberti, Mary,Bagadion, Alyssa,Li, Yubing,Baier, John,Tseung, Chi-Wah,Settles, A. Mark,Bai, Fang,Daliberti, Mary,Bagadion, Alyssa,Li, Yubing,Baier, John,Tseung, Chi-Wah,Settles, A. Mark,Xu, Miaoyun,Evans, Matthew M. S..

[17]Seed traits evaluation from long-term selection of kernel oil concentration in a high-oil maize population KYHO. Wang Hong-Wu,Hu Hai-Xiao,Song Tong-Ming,Chen Shao-Jiang,Wang Hong-Wu. 2012

[18]Proteome changes associated with dormancy release of Dongxiang wild rice seeds. Xu, Heng-Heng,Liu, Shu-Jun,Wang, Wei-Qing,Song, Song-Quan,Song, Shun-Hua,Moller, Ian Max.

[19]Mapping of quantitative trait loci controlling physico-chemical properties of rice grains (Oryza sativa L.). Li, ZF,Wan, JM,Xia, JF,Yano, M.

作者其他论文 更多>>