Expression of genes involved in progesterone receptor paracrine signaling and their effect on litter size in pigs

文献类型： 外文期刊

第一作者： Chen, Xiao

作者： Chen, Xiao;Fu, Jinluan;Wang, Aiguo;Chen, Xiao;Fu, Jinluan;Wang, Aiguo;Chen, Xiao

作者机构：

关键词： Expression;Implantation;Litter size;Pigs;SNPs

期刊名称：JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY （ 影响因子：5.032； 五年影响因子：5.922 ）

ISSN： 2049-1891

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： Background: Embryonic mortality during the period of implantation strongly affects litter size in pigs. Progesterone receptor (PGR) paracrine signaling has been recognized to play a significant role in embryonic implantation. IHH, NR2F2, BMP2, FKBP4 and HAND2 were proved to involve in PGR paracrine signaling. The objective of this study was to evaluate the expression of IHH, NR2F2, BMP2, FKBP4 and HAND2 in endometrium of pregnant sows and to further investigate these genes' effect on litter size in pigs. Real-time PCR, western blot and immunostaining were used to study target genes/proteins expression in endometrium in pigs. RFLP-PCR was used to detect single nucleotide polymorphisms (SNPs) of target genes. Results: The results showed that the mRNA and protein expression levels of IHH, NR2F2 and BMP2 were up-regulated during implantation period (P < 0.05 or P < 0.01). All target proteins were mainly observed in luminal epithelium and glandular epithelium. Interestingly, the staining of NR2F2 and HAND2 was also strong in stroma. SNPs detection revealed that there was a -204C > A mutation in promoter region of NR2F2 gene. Three genotypes were found in Large White, Landrace and Duroc sows. A total of 1847 litter records from 625 sows genotyped at NR2F2 gene were used to analyze the total number born (TNB) and number born alive (NBA). The study of the effect on litter size suggested that sows with genotype CC tend to have higher litter size. Conclusions: These results showed the expression patterns of genes/proteins involved in PGR paracrine signaling over implantation time. And the candidate gene for litter size was identified from genes involved in this signaling. This study could be a resource for further studies to identify the roles of these genes for embryonic implantation in pigs.

分类号：

  • 相关文献

[1]Molecular characterization and SNPs analysis of the porcine Deleted in AZoospermia Like (pDAZL) gene. Zhang, Y. H.,Niu, B. Y.,Ren, Z. Q.,Zuo, B.,Xu, D. Q.,Lei, M. G.,Zheng, R.,Jiang, S. W.,Deng, C. Y.,Xiong, Y. Z.,Li, F. E.,Zhang, Y. H.,Niu, B. Y.,Ren, Z. Q.,Zuo, B.,Xu, D. Q.,Lei, M. G.,Zheng, R.,Jiang, S. W.,Deng, C. Y.,Xiong, Y. Z.,Li, F. E.,Mei, S. Q.,Peng, X. W..

[2]Renaturation and purification of ApxII toxin of Actinobacillus pleuropneumoniae. Wang, Chunlai,Liu, Siguo,Peng, Yonggang,Shao, Meili,Wang, Yong,Gong, Qiang,Chang, Yuehong,Liu, Jiandong,Liu, Huifang,Liu, Di,Kong, Xiangang.

[3]Up-regulation of NLRC5 and NF-kappa B signaling pathway in carrier chickens challenged with Salmonella enterica Serovar Pullorum at different persistence periods. Liu, Xiangping,Sheng, Zhongwei,Dou, Xinhong,Wang, Kehua,Ma, Teng,Wang, Hongzhi,Li, Zhiteng,Pan, Zhiming,Chang, Guobin,Chen, Guohong. 2015

[4]Fecal progestin concentrations as an indicator of reproductive success in American Mink. Cao, Xinyan,Wei, Haijun,Xue, Hailong,Li, Xiaoxia,Zhao, Weigang,Xu, Chao,Wang, Shiyong,Diao, Yunfei,Xu, Baozeng,Cao, Xinyan,Wei, Haijun,Xue, Hailong,Li, Xiaoxia,Zhao, Weigang,Xu, Chao,Wang, Shiyong,Diao, Yunfei,Xu, Baozeng,Rose, Jack.

[5]Antler Transformation is Advanced by Inversion of Antlerogenic Periosteum Implants in Sika Deer (Cervus nippon). Gao, Xiuhua,Wang, Wenying,Li, Chunyi. 2010

[6]Differential expression and activation of Stat3 during mouse embryo implantation and decidualization. Teng, CB,Diao, HL,Ma, XH,Xu, LB,Yang, ZM.

[7]Broodstock rearing and controlled reproduction of reeves shad Tenualosa reevesii. Wang, HP,Xiong, BX,Wei, KJ,Yao, H.

[8]Heritabilities and genetic and phenotypic correlations of litter uniformity and litter size in Large White sows. Zhang Tian,Wang Li-gang,Shi Hui-bi,Yan Hua,Zhang Long-chao,Liu Xin,Pu Lei,Liang Jing,Zhang Yue-bo,Zhao Ke-bin,Wang Li-xian. 2016

[9]DNA Polymorphisms of 5 '-Flanking Region of Insulin-Like Growth Factor 1 Gene and Their Association with Reproduction Traits in Goats. Wang Ping-qing,Tan Ying,Zhang Bao-yun,Deng La-mei,Fan Qi,Liu Chong-xu,Chu Ming-xing. 2011

[10]DIFFERENTIAL EXPRESSION OF MIR-145, MIR-429 AND ITS TARGET GENES IN PARTIAL REPRODUCTIVE TISSUES OF SWINE WITH HIGH AND LOW LITTER SIZE. Zhang, Xiao-Dong,Feng, Yi-Fang,Zhang, Xu,Tian, Mi,Wu, Tao,Ye, Peng-Fei,Zhang, Wei,Ding, Yue-Yun,Yin, Zong-Jun,Zhang, Xiao-Dong,Feng, Yi-Fang,Zhang, Xu,Tian, Mi,Wu, Tao,Ye, Peng-Fei,Zhang, Wei,Ding, Yue-Yun,Yin, Zong-Jun,Chu, Ming-Xing. 2017

[11]Molecular cloning and expression pattern of the porcine 5-aminolevulinate synthase 1 (ALAS1) gene and its association with reproductive traits. Liu, L. Q.,Li, F. E.,Deng, C. Y.,Li, F. E.,Deng, C. Y.. 2016

[12]Polymorphism of bone morphogenetic protein 4 gene and its relationship with litter size of Jining Grey goats. Chu, M. X.,Feng, T.,Di, R.,Cao, G. L.,Fang, L.,Ma, Y. H.,Li, K.,Lu, L.,Wang, P. Q..

[13]Molecular cloning and polymorphism of the porcine H2AFZ gene. Zhang, Y. H.,Zuo, B.,Lei, M. G.,Xiong, Y. Z.,Li, F. E.,Zhang, Y. H.,Zuo, B.,Lei, M. G.,Xiong, Y. Z.,Li, F. E.,Mei, S. Q.,Peng, X. W.. 2009

[14]Predictive potential of microsatellite markers on heterosis of fecundity in crossbred sheep. Di, R.,Chu, M. X.,Fang, L.,Feng, T.,Cao, G. L.,Li, Y. L.,Chen, H. Q.,Zhang, L.,Li, X. W..

[15]Polymorphism of GDF9 gene and its association with litter size in goats. Chu, M. X.,Feng, T.,Cao, G. L.,Fang, L.,Di, R.,Huang, D. W.,Wu, Z. H.,Li, X. W.,Li, N..

[16]Analysis on DNA sequence of KiSS-1 gene and its association with litter size in goats. Cao, G. L.,Chu, M. X.,Fang, L.,Di, R.,Feng, T.,Li, N..

[17]Polymorphism of 5' regulatory region of ovine FSHR gene and its association with litter size in Small Tail Han sheep. Chu, M. X.,Huang, D. W.,Feng, T.,Cao, G. L.,Fang, L.,Di, R.,Tang, Q. Q.,Ma, Y. H.,Li, K.,Guo, X. H.,Guo, X. H.,Feng, C. J.,Li, Y..

[18]Association between PCR-SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey goats. Chu, M. X.,Jiao, C. L.,He, Y. Q.,Wang, J. Y.,Liu, Z. H.,Chen, G. H..

[19]Analysis on DNA sequence of goat RFRP gene and its possible association with average daily sunshine duration. Huang, D. W.,Di, R.,Wang, J. X.,Chu, M. X.,He, J. N.,Cao, G. L.,Fang, L.,Feng, T.,Li, N..

[20]Polymorphisms of caprine GDF9 gene and their association with litter size in Jining Grey goats. Feng, T.,Chu, M. X.,Cao, G. L.,Di, R.,Fang, L.,Geng, C. X.,Chen, H. Q.,Lang, X. Z.,Liu, X. L.,Li, N..

作者其他论文 更多>>

微信小程序