Applications of Replicating-Competent Reporter-Expressing Viruses in Diagnostic and Molecular Virology

文献类型： 外文期刊

第一作者： Li, Yongfeng

作者： Li, Yongfeng;Li, Lian-Feng;Yu, Shaoxiong;Wang, Xiao;Zhang, Lingkai;Yu, Jiahui;Xie, Libao;Qiu, Hua-Ji;Li, Weike;Ali, Razim

作者机构：

关键词： replicating-competent virus;reporter;high-throughput screening;molecular virology

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN： 1999-4915

年卷期： 2016 年 8 卷 5 期

页码：

收录情况： SCI

摘要： Commonly used tests based on wild-type viruses, such as immunostaining, cannot meet the demands for rapid detection of viral replication, high-throughput screening for antivirals, as well as for tracking viral proteins or virus transport in real time. Notably, the development of replicating-competent reporter-expressing viruses (RCREVs) has provided an excellent option to detect directly viral replication without the use of secondary labeling, which represents a significant advance in virology. This article reviews the applications of RCREVs in diagnostic and molecular virology, including rapid neutralization tests, high-throughput screening systems, identification of viral receptors and virus-host interactions, dynamics of viral infections in vitro and in vivo, vaccination approaches and others. However, there remain various challenges associated with RCREVs, including pathogenicity alterations due to the insertion of a reporter gene, instability or loss of the reporter gene expression, or attenuation of reporter signals in vivo. Despite all these limitations, RCREVs have become powerful tools for both basic and applied virology with the development of new technologies for generating RCREVs, the inventions of novel reporters and the better understanding of regulation of viral replication.

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