The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background

文献类型： 外文期刊

第一作者： Wang, Shuang

作者： Wang, Shuang;Zhang, Xiang Xiang;Ji, Wei;Zhao, Yue;Li, Jie;Song, Fuping

作者机构：

关键词： Aspergillus niger;amyR;Regulation;Proteomics;Transcriptome

期刊名称：MICROBIAL CELL FACTORIES （ 影响因子：5.328； 五年影响因子：5.588 ）

ISSN： 1475-2859

年卷期： 2016 年 15 卷

页码：

收录情况： SCI

摘要： Background: The filamentous fungus Aspergillus niger is widely exploited as an important expression host for industrial production. The glucoamylase high-producing strain A. niger CICC2462 has been used as a host strain for the establishment of a secretion expression system. It expresses recombinant xylanase, mannase and asparaginase at a high level, but some high secretory background proteins in these recombinant strains still remain, such as alphaamylase and alpha-glucosidase; lead to a low-purity of fermentation products. The aim was to construct an A. niger host strain with a low background of protein secretion. Results: The transcription factor amyR was deleted in A. niger CICC2462, and the results from enzyme activity assays and SDS-PAGE analysis showed that the glucoamylase and amylase activities of the Delta amyR strains were significantly lower than those of the wild-type strain. High-throughput RNA-sequencing and shotgun LC-MS/ MS proteomic technology analysis demonstrated that the expression of amylolytic enzymes was decreased at both the transcriptional and translational levels in the Delta amyR strain. Interestingly, the Delta amyR strain growth rate better than the wild-type strain. Conclusions: Our findings clearly indicated that the Delta amyR strain of A. niger CICC2462 can be used as a host strain with a low background of protein secretion.

分类号：

  • 相关文献

[1]De Novo Assembly and Characterization of the Transcriptome of the Chinese Medicinal Herb, Gentiana rigescens. Zhang, Xiaodong,Li, Caixia,Allan, Andrew C.,Wang, Yuanzhong,Yao, Qiuyang. 2015

[2]Mechanism of Glucose Regulates the Fruiting Body Formation in the Beech Culinary-Medicinal Mushroom, Hypsizygus marmoreus (Agaricomycetes). Zhang, Jin-Jing,Chen, Hui,Xie, Min-Ying,Chen, Ming-Jie,Hao, Hai-Bo,Wang, Hong,Feng, Zhi-Yong,Zhang, Jin-Jing,Xie, Min-Ying,Hao, Hai-Bo,Feng, Zhi-Yong.

[3]Differential gene expression and associated QTL mapping for cotton yield based on a cDNA-AFLP transcriptome map in an immortalized F-2. Liu, Renzhong,Wang, Baohua,Guo, Wangzhen,Zhang, Tianzhen,Liu, Renzhong,Wang, Liguo.

[4]The biocontrol effect of Sporidiobolus pararoseus Y16 against postharvest diseases in table grapes caused by Aspergillus niger and the possible mechanisms involved. Li, Qiaofei,Li, Chaolan,Zhang, Hongyin,Zhang, Xiaoyun,Zheng, Xiangfeng,Yang, Qiya,Apaliya, Maurice Tibiru,Boateng, Nana Adwoa Serwah,Sun, Yiwen,Li, Pengxia. 2017

[5]Aspergillus leaf spot of field bindweed (Convolvulus arvensis L.) caused by Aspergillus niger in China. Zhang, Xuekun,Lin, Kejian,Liu, Zheng,Yu, Yu,Sun, Yan,Zhao, Jing,Xi, Hui. 2016

[6]Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization. Liu Xue,Zhu Chang-xiong,Liu Xue,Delvigne, Frank,Gong Ming-bo. 2017

[7]Efficient Expression of an Acidic Endo-polygalacturonase from Aspergillus niger and Its Application in Juice Production. Wang, Jiaojiao,Zhang, Yuhong,Qin, Xing,Zhang, Deqing,Li, Jinyang,Zhang, Wei,Wang, Jiaojiao,Huang, He,Gao, Lingyu,Han, Bin.

[8]Aflatoxin B-1 inhibition in Aspergillus flavus by Aspergillus niger through down-regulating expression of major biosynthetic genes and AFB(1) degradation by atoxigenic A. flavus. Xing, Fuguo,Wang, Limin,Liu, Xiao,Selvaraj, Jonathan Nimal,Wang, Yan,Zhao, Yueju,Liu, Yang.

[9]Phosphate solubilization and promotion of maize growth by Penicillium oxalicum P4 and Aspergillus niger P85 in a calcareous soil. Yin, Zhongwei,Shi, Fachao,Jiang, Hongmei,Fan, Bingquan,Yin, Zhongwei,Chen, Sanfeng,Roberts, Daniel P..

[10]Preparation and utilization of phosphate biofertilizers using agricultural waste. Wang Hong-yuan,Liu Shen,Zhai Li-mei,Zhang Ji-zong,Ren Tian-zhi,Fan Bing-quan,Liu Hong-bin. 2015

[11]Pectinase production by Aspergillus niger using wastewater in solid state fermentation for eliciting plant disease resistance. Bai, ZH,Zhang, HX,Qi, HY,Peng, XW,Li, BJ.

[12]Complexation of synthetic CDM-AM copolymer with natamycin and carbendazim to improve solubility and fungicidal activity. Li, Yong-Fu,Ji, Jing,Guo, Qin,Ha, Yi-Ming,Li, Qing-Peng,Li, Yong-Fu,Ji, Jing,Guo, Qin,Ha, Yi-Ming,Li, Qing-Peng.

[13]An acidic pectin lyase from Aspergillus niger with favourable efficiency in fruit juice clarification. Xu, S. X.,Qin, X.,Wu, K.,Qin, X.,Liu, B.,Zhang, D. Q.,Zhang, W.,Zhang, Y. H..

[14]Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a truncated version of -glucosidase from Aspergillus niger. Li, Zhenjun,Han, Hongjuan,Wang, Bo,Gao, Jianjie,Zhu, Bo,Peng, Rihe,Yao, Quanhong.

[15]Asperpyrone-Type Bis-Naphtho--Pyrones with COX-2-Inhibitory Activities from Marine-Derived Fungus Aspergillus niger. Fang, Wei,Lin, Xiuping,Liu, Yonghong,Zhou, Xuefeng,Wang, Jianjiao,Tao, Huaming.

[16]Structure-based fragment shuffling of two fungal phytases for combination of desirable properties. Wang, Xunzhang,Bei, Jinlong,Chen, Zhuang,Jiang, Zongyong.

[17]Genome Shuffling of Aspergillus niger for Improving Transglycosylation Activity. Chen, Guiguang,Gu, Lingli,Zeng, Wei,Liang, Zhiqun,Li, Wei.

[18]Aspergillus niger Enhance Bioactive Compounds Biosynthesis As Well As Expression of Functional Genes in Adventitious Roots of Glycyrrhiza uralensis Fisch. Li, Jing,Wang, Juan,Li, Jinxin,Li, Hongfa,Gao, Wenyuan,Li, Jing,Liu, Dahui,Li, Jianli,Liu, Shujie.

[19]Aspernigrins with anti-HIV-1 activities from the marine-derived fungus Aspergillus niger SCSIO Jcsw6F30. Zhou, Xuefeng,Lin, Xiuping,Yang, Bingjie,Liu, Yonghong,Fang, Wei,Tan, Suiyi,Xun, Tianrong,Yang, Bingjie,Liu, Shuwen.

[20]Improvement of glucoamylase production for raw-starch digestion in Aspergillus niger F-01 by maltose stearic acid ester. Sun, Haiyan,Peng, Ming,Sun, Haiyan,Peng, Ming.

作者其他论文 更多>>

微信小程序