Construction and Validation of a Dual-Transgene Vector System for Stable Transformation in Plants

文献类型： 外文期刊

第一作者： He, Zhimin

作者： He, Zhimin;He, Reqing;Yan, Jindong;Zhong, Ming;Zhao, Xiaoying;Liu, Xuanming;He, Zhimin;Wang, Xu;Liu, Bin;Bian, Mingdi;Liu, Xuanming

作者机构：

关键词： Co-expression;Dual-transgene vector;pDT1;pDT7;pDT7G

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：4.275； 五年影响因子：5.223 ）

ISSN： 1673-8527

年卷期： 2016 年 43 卷 4 期

页码：

收录情况： SCI

摘要： In this study, we constructed dual-transgene vectors (pDT1, pDT7, and pDT7G) that simultaneously co-expressed two genes in plants. ACTIN2 and UBQ10 promoters were used to control the expression of these two genes. The 4 x Myc, 3 x HA, and 3 x Flag reporter genes allowed for the convenient identification of a tunable co-expression system in plants, whereas the dexamethasone (Dex) inducible reporter gene C-terminus of the glucocorticoid receptor (cGR) provided Dex-dependent translocation of the fusion gene between the nucleus and cytoplasm. The function of pDT vectors was validated using four pairwise genes in Nicotiana benthamiana or Arabidopsis thaliana. The co-expression efficiency of two genes from the pDT1 and pDT7G vectors was 35% and 42%, respectively, which ensured the generation of sufficient transgenic materials. These pDT vectors are simple, reliable, efficient, and time-saving tools for the co-expression of two genes through a single transformation event and can be used in the study of protein-protein interactions or multi-component complexes.

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