Evaluation of different nested PCRs for detection of Anaplasma phagocytophilum in ruminants and ticks

文献类型： 外文期刊

第一作者： Yang, Jifei

作者： Yang, Jifei;Liu, Zhijie;Niu, Qingli;Liu, Junlong;Xie, Jingying;Chen, Qiuyu;Chen, Ze;Guan, Guiquan;Liu, Guangyuan;Luo, Jianxun;Yin, Hong;Yin, Hong

作者机构：

关键词： A. phagocytophilum;Diagnosis;PCR;Ruminants;Tick;Prevalence

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2016 年 12 卷

页码：

收录情况： SCI

摘要： Background: Anaplasma phagocytophilum is a causative agent of granulocytic anaplasmosis in mammals, which has a broad geographical distribution and a high degree of clinical diversity. Currently, numerous PCR assays have been developed and used for the detection of A. phagocytophilum in various specimens. However, their performance varies. The aim of this study was to evaluate the performance of five nested PCR assays by detection of 363 ruminant and tick samples, and to select the most appropriate methods for the sensitive detection of A. phagocytophilum in environmental or clinical samples. Results: Positive PCR results for A. phagocytophilum were obtained in 75 (20.7 %), 42 (11.6 %) and 19 (5.2 %) specimens with primer sets EC (EC9/EC12a and SSAP2f/SSAP2r), EE (EE1/EE2 and EE3/EE4) and ge (ge3a/ge10r, ge9f/ge2), respectively. The amplification of template DNA with the primer set MSP (MAP4AP5/MSP4AP3, msp4f/msp4r) could not be obtained in both ruminants and ticks, and a low specificity of the EL primers [EL(569) F/EL(1193) R, EL(569) F/ EL(1142) R] in tick samples was observed. Our results revealed that the nested PCR with primer set EC complementary to the 16S rRNA gene was the most sensitive assay for detection of A. phagocytophilum in ruminant and tick specimens. A. phagocytophilum was detected in 47 (35.1 %) sheep, 12 (10.4 %) cattle, and 17 (14.9 %) ticks. Two A. phagocytophilum genotypes were identified, that varied between sheep and cattle in sample collection sites. Conclusions: This report provides more valuable information for the diagnosis and management of granulocytic anaplasmosis in China.

分类号：

  • 相关文献

[1]Evaluation of molecular methods for detection of Borrelia burgdorferi senso lato in ticks. Yang, Jifei,Liu, Zhijie,Guan, Guiquan,Che, Rongxuan,Niu, Qingli,Li, Youquan,Liu, Junlong,Ma, Miling,Ren, Qiaoyun,Liu, Aihong,Luo, Jianxun,Yin, Hong.

[2]DIFFERENTIAL DIAGNOSIS OF MONIEZIA BENEDENI AND M. EXPANSA (ANOPLOCEPHALIDAE) BY PCR USING MARKERS IN SMALL RIBOSOMAL DNA (18S rDNA). Yan, HongBin,Liu, Youyu,Lou, Zhongzi,Ni, XingWei,Jia, WanZhong,Bo, XinWen,Shi, WanGui,Zhan, Fang,Ooi, HongKean,Ooi, HongKean.

[3]Prevalence of porcine circovirus-like agent P1 in Jiangsu, China. Wen, Libin,He, Kongwang,Yu, Zhengyu,Mao, Aihua,Zhong, Shulin,Ni, Yanxiu,Zhang, Xuehan,Li, Bin,Wang, Xiaomin,Zhou, Junming,Guo, Rongli,Lv, Lixin,Jiang, Jieyuan,Yang, Hanchun. 2011

[4]Prevalence of Toxoplasma gondii antibodies, circulating antigens and DNA in stray cats in Shanghai, China. Wang, Quan,Jiang, Wei,Chen, Yong-Jun,Liu, Chun-Ying,Shi, Jin-lei,Li, Xin-tong. 2012

[5]Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle. Hou, Qinlei,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Li, Liming,Wang, Changfa,Sun, Tao,Wang, Lingling,Hou, Minghai,Zhong, Jifeng,Hang, Suqin.

[6]Bacterial community composition and fermentation in the rumen of Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries). Qian, Wenxi,Wu, JianPing,Qian, Wenxi,Ao, Weiping,Li, ZhiPeng,Li, Guangyu,Zhao, Guangyong.

[7]Prevalence of Theileria infections in goats and sheep in southeastern China. Yin, Hong.

[8]Xuhuai Goat H-FABP Gene Clone, Subcellular Localization of Expression Products and the Preparation of Transgenic Mice. Yin, Yan-hui,Li, Bi-chun,Wei, Guang-hui,Zhu, Cai-ye,Li, Wei,Zhang, Ya-ni,Du, Li-xin,Cao, Wen-guang.

[9]The relationship between intestinal digestibility of crude protein and dry matters and the protein fractions with ruminant feedstuffs. Zhou, R.,Wang, J. Q.,Pan, F. M.,Bu, D. P.,Wei, H. Y.,Zhou, L. Y.. 2010

[10]Molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from China evaluated by nested PCRs. Yu, Pei-Fa,Niu, Qing-Li,Liu, Zhi-Jie,Yang, Ji-Fei,Chen, Ze,Guan, Gui-Quan,Liu, Guang-Yuan,Luo, Jian-Xun,Yin, Hong,Yin, Hong. 2016

[11]Epidemiology of toxoplasmosis: role of the tick Haemaphysalis longicornis. Zhou, Yongzhi,Zhang, Houshuang,Cao, Jie,Gong, Haiyan,Zhou, Jinlin,Zhou, Jinlin. 2016

[12]Novel spotted fever group rickettsiae in Haemaphysalis qinghaiensis ticks from Gansu, Northwest China. Yang, Jifei,Tian, Zhancheng,Liu, Zhijie,Niu, Qingli,Han, Rong,Li, Youquan,Guan, Guiquan,Liu, Junlong,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong. 2016

[13]First report on the occurrence of Theileria sp OT3 in China. Tian, Zhancheng,Liu, Guangyuan,Yin, Hong,Xie, Junren,Wang, Suyan,Yuan, Xiaosong,Wang, Fangfang,Luo, Jin. 2014

[14]Cloning and characterization of a cDNA clone encoding troponin T from tick Haemaphysalis qinghaiensis (Acari: Ixodidae). Gao, Jinliang,Luo, Jianxun,Fan, Ruiquan,Guan, Guiquan,Yin, Hong,Gao, Jinliang,Fingerle, Volker,Sugimoto, Chihiro,Inoue, Noboru.

[15]Identification and characterization of microRNAs by deep-sequencing in Hyalomma anatolicum anatolicum (Acari: Ixodidae) ticks. Luo, Jin,Liu, Guang-Yuan,Chen, Ze,Ren, Qiao-Yun,Yin, Hong,Luo, Jian-Xun,Wang, Hui,Yin, Hong,Wang, Hui,Wang, Hui.

[16]Evaluation on Infectivity of Babesia microti to Domestic Animals and Ticks Outside the Ixodes Genus. Wu, Jiajun,Cao, Jie,Zhou, Yongzhi,Zhang, Houshuang,Gong, Haiyan,Zhou, Jinlin,Zhou, Jinlin. 2017

[17]Seroprevalence survey of Babesia gibsoni infection and tick species in dogs in East China. Cao, Jie,Yang, Qiqing,Zhou, Yongzhi,Zhang, Houshuang,Gong, Haiyan,Zhou, Jinlin,Zhang, Jianwei,Zhou, Jinlin.

[18]Primary characterization of a putative novel TBC1 domain family member 13 from Haemaphysalis qinghaiensis. Tian, Zhancheng,Du, Junzheng,Gao, Shandian,Yang, Jifei,Luo, Jin,Xing, Shanshan,Du, Xiaoyue,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong.

[19]Biotechnological advances in the diagnosis, species differentiation and phylogenetic analysis of Schistosoma spp.. Zhao, Guang-Hui,Li, Juan,Li, Xiao-Yan,Zhu, Xing-Quan,Zhao, Guang-Hui,Li, Juan,Lin, Rui-Qing,Blair, David,Li, Xiao-Yan,Elsheikha, Hany M.,Zou, Feng-Cai,Zhu, Xing-Quan. 2012

[20]Monoclonal antibodies for differentiating infections of three serological-related tospoviruses prevalent in Southwestern China. Chen, Yu-Han,Chien, Wan-Chu,Sun, Jing-Hua,Wang, Yun-Chi,Chen, Tsung-Chi,Dong, Jiahong,Zheng, Kuanyu,Wu, Kuo,Yeh, Shyi-Dong,Yeh, Shyi-Dong,Chen, Tsung-Chi. 2016

作者其他论文 更多>>

微信小程序