Identification and Characterization of a Glyoxalase I Gene in a Rapeseed Cultivar with Seed Thermotolerance

文献类型： 外文期刊

第一作者： Yan, Guixin

作者： Yan, Guixin;Lv, Xiaodan;Gao, Guizhen;Li, Feng;Li, Jun;Qiao, Jiangwei;Xu, Kun;Chen, Biyun;Wang, Limin;Xiao, Xin;Wu, Xiaoming

作者机构：

关键词： Glyoxalase I gene;seed thermotolerance;proteomics;Brassica napus L.;yeast

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： Glyoxalase I (GLYI) is a ubiquitous enzyme in all organisms that catalyzes the conversion of the potent cytotoxin methylglyoxal to S-D-lactoylglutathione. Although many reports suggest the importance of GLYI in the plant response to stress, its function in seeds requires further study. Here, we identified a heat -induced GLYI from Brassica napus seeds, BnGLYI, using a comparative proteomics approach. Two-dimensional gel analyses revealed that BnGLYI protein expression upon heat treatment was significantly elevated in thermotolerant seeds but was diminished in heat -sensitive seeds. The BnGLYI-2 and BnGLYI-3 genes from the heat -sensitive and thermotolerant cultivars, respectively, were characterized, and analyzed. Only two amino acid residue variations were found between the amino acid sequences of the two genes. Moreover, overexpressing BnGLYI-3 in yeast cells enhanced tolerance to heat and cold stress and significantly increased GLYI activity compared to overexpressing BnGLYI-2. In addition, BnGLYI-3 transformants showed enhanced superoxide dismutase activities under heat and cold treatment, whereas these activities were diminished for BnGLYI-2 transformants. Taken together, these results indicate that overexpression of the BnGLYI3 gene imparts thermotolerance and cold tolerance in yeast and that the variations in BnGLYI-3 may play an important role in the responses to temperature stresses.

分类号：

  • 相关文献

[1]Microbial Diversity and Biochemical Analysis of Suanzhou: A Traditional Chinese Fermented Cereal Gruel. Qin, Huibin,Sun, Qinghui,Pan, Xuewei,Yang, Hongjiang,Qin, Huibin,Qiao, Zhijun. 2016

[2]Effects of different forms of yeast Saccharomyces cerevisiae on growth performance, intestinal development, and systemic immunity in early-weaned piglets. Jiang, Zongyong,Wei, Shaoyong,Wang, Zhilin,Hu, Shenglan,Zheng, Chuntian,Hu, Youjun,Wang, Li,Ma, Xianyong,Yang, Xuefen,Jiang, Zongyong,Zhu, Cui,Chen, Zhuang. 2015

[3]Brassica napus soluble epoxide hydrolase (BNSEH1) - Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris. Bellevik, S,Zhang, JM,Meijer, J. 2002

[4]Attraction of Coffee Bean Weevil, Araecerus fasciculatus, to Volatiles from the Industrial Yeast Kluyveromyces lactis. Mei, Xiang-Dong,She, Dongmei,Ning, Jun,Zhang, Xiao-Fang,Li, Yao-Fa,Zhang, Tao.

[5]High level expression of a recombinant acid phytase gene in Pichia pastoris. Xiong, AS,Yao, QH,Peng, RH,Han, PL,Cheng, ZM,Li, Y.

[6]Du1, encoding a novel Prp1 protein, regulates starch biosynthesis through affecting the splicing of Wx(b) supercript stop pre-mRNAs in rice (Oryza sativa L.). Zeng, Dali,Yan, Meixian,Wang, Yonghong,Liu, Xinfang,Qian, Qian,Li, Jiayang.

[7]Identification and characterization of PtDGAT2B, an acyltransferase of the DGAT2 acyl-Coenzyme A: Diacylglycerol acyltransferase family in the diatom Phaeodactylum tricornutum. Gong, Yangmin,Zhang, Junping,Guo, Xiaojing,Wan, Xia,Liang, Zhuo,Hu, Chuan Jiong,Jiang, Mulan,Zhang, Junping.

[8]Cloning and expression analysis of a vacuolar Na+/H+ antiporter gene from Alfalfa. Yang, QC,Wu, MS,Wang, PQ,Kang, JM,Zhou, XL.

[9]Production and Characteristics of High Quality Vinegar from Sugarcane Juice. Lin, Bo,Li, Yang-Rui,Zheng, Feng-Jin,Sun, Jian,Li, Zhi-Chun. 2015

[10]Cloning and Functional Characterization of a Fatty Acyl-Acyl Carrier Protein Thioesterase Gene (BnFatB) in Brassica napus L.. Tan, X. L.,Huang, Q.,Tan, R. K.,Zhang, Zh. Y.,Wang, Zh.,Li, X. F.,Wu, L.,Lu, Ch. M.. 2015

[11]Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents. Tan Xiao-li,Zheng Xiang-feng,Zhang Zhi-yan,Wang Zheng,Xia Heng-chuan,Gu Shou-lai,Lu Chang-ming. 2014

[12]Prey diet quality affects predation, oviposition and conversion rate of the predatory mite Neoseiulus barkeri (Acari: Phytoseiidae). Yang, Kang,Wang, Endong,Xu, Xuenong.

[13]Characterization of a yeast recombinant laccase rLAC-EN3-1 and its application in decolorizing synthetic dye with the coexistence of metal ions and organic solvents. Zhuo, Rui,He, Feng,Zhang, Xiaoyu,Yang, Yang,Zhuo, Rui,Yang, Yang.

[14]Identification and biochemical characterization of five long-chain acyl-coenzyme A synthetases from the diatom Phaeodactylum tricornutum. Guo, Xiaojing,Jiang, Mulan,Wan, Xia,Hu, Chuanjiong,Gong, Yangmin.

[15]An optimized, highly efficient, self-assembled, subvirus-like particle of infectious bursal disease virus (IBDV). Wang, Miao,Pan, Qing,Lu, Zhen,Li, Kai,Gao, Honglei,Qi, Xiaole,Gao, Yulong,Wang, Xiaomei,Wang, Xiaomei.

[16]Preparation and Characteristics of Sugarcane Low Alcoholic Drink by Submerged Alcoholic Fermentation. Lin, Bo,Wang, Tian-Shun,Li, Yang-Rui,Zheng, Feng-Jin. 2013

[17]Generation of High Titre Antibodies to Pokeweed Antiviral Protein (PAP) in Rabbits Using Synthetic Peptides and their Use in Detecting PAP in Transgenic Petunia and Yeast. Li, Yu,Wang, Xifeng. 2012

[18]Characterization of a laccase gene from the white-rot fungi Trametes sp 5930 isolated from Shennongjia Nature Reserve in China and studying on the capability of decolorization of different synthetic dyes. Yang, Yang,Ma, Fuying,Yu, Hongbo,Fan, Fangfang,Zhang, Xiaoyu,Yang, Yang,Wan, Xia,Jiang, Mulan. 2011

[19]Characterization of a cold responsive HbICE1 gene from rubber trees. Deng, Xiao Min,Wang, Jian Xiao,Li, Yan,Wang, Jing,Tian, Wei-Min,Deng, Xiao Min,Wang, Jian Xiao,Tian, Wei-Min.

[20]Effects of dietary live and heat-inactive baker's yeast on growth, gut health, and disease resistance of Nile tilapia under high rearing density. Ran, Chao,Huang, Lu,Hu, Jun,He, Suxu,Li, Zhimin,Wang, Yibing,Liu, Zhi,Xu, Li,Yang, Yalin,Zhou, Zhigang,Tacon, Philippe.

作者其他论文 更多>>

微信小程序