Newcastle Disease Virus V Protein Targets Phosphorylated STAT1 to Block IFN-I Signaling

文献类型： 外文期刊

第一作者： Qiu, Xusheng

作者： Qiu, Xusheng;Fu, Qiang;Meng, Chunchun;Yu, Shengqing;Zhan, Yuan;Dong, Luna;Song, Cuiping;Sun, Yingjie;Tan, Lei;Ding, Chan;Fu, Qiang;Hu, Shunlin;Wang, Xiaoquan;Liu, Xiaowen;Peng, Daxin;Liu, Xiufan;Peng, Daxin;Liu, Xiufan;Ding, Chan

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2016 年 11 卷 2 期

页码：

收录情况： SCI

摘要： Newcastle disease virus (NDV) V protein is considered as an effector for IFN antagonism, however, the mechanism remains unknown. In this study, the expression of STAT1 and phospho-STAT1 in cells infected with NDV or transfected with V protein-expressing plasmids were analyzed. Our results showed that NDV V protein targets phospho-STAT1 reduction in the cells depends on the stimulation of IFN-alpha. In addition, a V-deficient genotype VII recombinant NDV strain rZJ1-VS was constructed using reverse genetic technique to confirm the results. The rZJ1-VS lost the ability to reduce phospho-STAT1 and induced higher expression of IFN-responsive genes in infected cells. Furthermore, treatment with an ubiquitin E1 inhibitor PYR-41 demonstrated that phospho-STAT1 reduction was caused by degradation, but not de-phosphorylation. We conclude that NDV V protein targets phospho-STAT1 degradation to block IFN-alpha signaling, which adds novel knowledge to the strategies used by paramyxoviruses to evade IFN.

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