Functional Genomic Analysis of Aspergillus flavus Interacting with Resistant and Susceptible Peanut

文献类型： 外文期刊

第一作者： Wang, Houmiao

作者： Wang, Houmiao;Lei, Yong;Yan, Liying;Wan, Liyun;Ren, Xiaoping;Chen, Silong;Jiang, Huifang;Liao, Boshou;Wang, Houmiao;Lei, Yong;Yan, Liying;Wan, Liyun;Ren, Xiaoping;Chen, Silong;Jiang, Huifang;Liao, Boshou;Dai, Xiaofeng;Guo, Wei

作者机构：

关键词： Aspergillus;flavus;peanut;interaction;aflatoxin biosynthesis;RNA-seq

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2016 年 8 卷 2 期

页码：

收录情况： SCI

摘要： In the Aspergillusflavus (A. flavus)-peanut pathosystem, development and metabolism of the fungus directly influence aflatoxin contamination. To comprehensively understand the molecular mechanism of A. flavus interaction with peanut, RNA-seq was used for global transcriptome profiling of A. flavus during interaction with resistant and susceptible peanut genotypes. In total, 67.46 Gb of high-quality bases were generated for A. flavus-resistant (af_R) and -susceptible peanut (af_S) at one (T1), three (T2) and seven (T3) days post-inoculation. The uniquely mapped reads to A. flavus reference genome in the libraries of af_R and af_S at T2 and T3 were subjected to further analysis, with more than 72% of all obtained genes expressed in the eight libraries. Comparison of expression levels both af_R vs. af_S and T2 vs. T3 uncovered 1926 differentially expressed genes (DEGs). DEGs associated with mycelial growth, conidial development and aflatoxin biosynthesis were up-regulated in af_S compared with af_R, implying that A. flavus mycelia more easily penetrate and produce much more aflatoxin in susceptible than in resistant peanut. Our results serve as a foundation for understanding the molecular mechanisms of aflatoxin production differences between A. flavus-R and -S peanut, and offer new clues to manage aflatoxin contamination in crops.

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